PLANT-GROWTH PROMOTING RHIZOBACTERIA - STRAIN IDENTIFICATION BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS

被引:5
作者
BROWN, G
KHAN, Z
LIFSHITZ, R
机构
[1] Microbial Inoculants, Allelix Crop Technologies, Mississauga, Ont. L4V 1P1
关键词
Pseudomonads; Restriction fragment length polymorphism; Rhzobacteria; Strain identification;
D O I
10.1139/m90-042
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A genomic library of the Pseudomonas putida strain GR12-2 was screened to identify both genus-universal and strain-specific 8-kilobase inserts. The genus-universal clone (pAM141), in combination with the restriction enzymes EcoRI, PstI, and PvuII, was used to generate unique restriction fragment length polymorphisms for 20 related plant growth promoting rhizobacteria and seven reference strains. Strain restriction fragment length polymorphism profiles based on the genus-universal clone pAM141 allow positive identification of individual pseudomonad strains. The strain-specific clone (pAM227) clearly distinguished the parent strain (GR12-2) from 16 other pseudomonad strains, including 8 P. putida, 7 P. fluorescens, and 1 P. cepacia. pAM227 may be useful for monitoring the fate of the P. putida strain GR12-2 in the environment.
引用
收藏
页码:242 / 248
页数:7
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