THE CONSERVED U.G PAIR IN THE 5' SPLICE SITE DUPLEX OF A GROUP-I INTRON IS REQUIRED IN THE 1ST BUT NOT THE 2ND STEP OF SELF-SPLICING

被引：69
作者：
BARFOD, ET [1 ]
CECH, TR [1 ]
机构：
[1] UNIV COLORADO, HOWARD HUGHES MED INST, DEPT CHEM & BIOCHEM, BOULDER, CO 80309 USA
来源：
MOLECULAR AND CELLULAR BIOLOGY | 1989年 / 9卷 / 09期
关键词：
D O I：
10.1128/MCB.9.9.3657
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
引用
收藏
页码：3657 / 3666
页数：10
相关论文
共 36 条
[1]   DELETION OF NONCONSERVED HELICES NEAR THE 3' END OF THE RIBOSOMAL-RNA INTRON OF TETRAHYMENA-THERMOPHILA ALTERS SELF-SPLICING BUT NOT CORE CATALYTIC ACTIVITY [J].
BARFOD, ET ;
CECH, TR .
GENES & DEVELOPMENT, 1988, 2 (06) :652-663
[2]   SPECIFIC INTERACTION BETWEEN THE SELF-SPLICING RNA OF TETRAHYMENA AND ITS GUANOSINE SUBSTRATE - IMPLICATIONS FOR BIOLOGICAL CATALYSIS BY RNA [J].
BASS, BL ;
CECH, TR .
NATURE, 1984, 308 (5962) :820-826
[3]   ONE BINDING-SITE DETERMINES SEQUENCE SPECIFICITY OF TETRAHYMENA PRE-RIBOSOMAL-RNA SELF-SPLICING, TRANSSPLICING, AND RNA ENZYME-ACTIVITY [J].
BEEN, MD ;
CECH, TR .
CELL, 1986, 47 (02) :207-216
[4]   SELECTION OF CIRCULARIZATION SITES IN A GROUP-1 IVS RNA REQUIRES MULTIPLE ALIGNMENTS OF AN INTERNAL TEMPLATE-LIKE SEQUENCE [J].
BEEN, MD ;
CECH, TR .
CELL, 1987, 50 (06) :951-961
[5]   SITES OF CIRCULARIZATION OF THE TETRAHYMENA RIBOSOMAL-RNA IVS ARE DETERMINED BY SEQUENCE AND INFLUENCED BY POSITION AND SECONDARY STRUCTURE [J].
BEEN, MD ;
CECH, TR .
NUCLEIC ACIDS RESEARCH, 1985, 13 (23) :8389-8408
[6]   STRUCTURES INVOLVED IN TETRAHYMENA RIBOSOMAL-RNA SELF-SPLICING AND RNA ENZYME-ACTIVITY [J].
BEEN, MD ;
BARFOD, ET ;
BURKE, JM ;
PRICE, JV ;
TANNER, NK ;
ZAUG, AJ ;
CECH, TR .
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1987, 52 :147-157
[7]   RNA AS AN RNA-POLYMERASE - NET ELONGATION OF AN RNA PRIMER CATALYZED BY THE TETRAHYMENA RIBOZYME [J].
BEEN, MD ;
CECH, TR .
SCIENCE, 1988, 239 (4846) :1412-1416
[8]   CONSERVED SEQUENCES AND STRUCTURES OF GROUP-I INTRONS - BUILDING AN ACTIVE-SITE FOR RNA CATALYSIS - A REVIEW [J].
CECH, TR .
GENE, 1988, 73 (02) :259-271
[9]   INVITRO SPLICING OF THE RIBOSOMAL-RNA PRECURSOR OF TETRAHYMENA - INVOLVEMENT OF A GUANOSINE NUCLEOTIDE IN THE EXCISION OF THE INTERVENING SEQUENCE [J].
CECH, TR ;
ZAUG, AJ ;
GRABOWSKI, PJ .
CELL, 1981, 27 (03) :487-496
[10]   THE CHEMISTRY OF SELF-SPLICING RNA AND RNA ENZYMES [J].
CECH, TR .
SCIENCE, 1987, 236 (4808) :1532-1539
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