3-DIMENSIONAL STRUCTURE OF EXTENDED CHROMATIN FIBERS AS REVEALED BY TAPPING-MODE SCANNING FORCE MICROSCOPY

被引：195
作者：
LEUBA, SH
YANG, GL
ROBERT, C
SAMORI, B
VANHOLDE, K
ZLATANOVA, J
BUSTAMANTE, C
机构：
[1] UNIV OREGON,HOWARD HUGHES MED INST,EUGENE,OR 97403
[2] UNIV OREGON,INST MOLEC BIOL,EUGENE,OR 97403
[3] UNIV OREGON,DEPT CHEM,EUGENE,OR 97403
[4] OREGON STATE UNIV,DEPT BIOCHEM & BIOPHYS,CORVALLIS,OR 97331
[5] UNIV CALABRIA,DIPARTIMENTO CHIM,I-87036 COSENZA,ITALY
[6] BULGARIAN ACAD SCI,INST GENET,BU-1113 SOFIA,BULGARIA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 24期
关键词：
CHICKEN ERYTHROCYTES; MODELING;
D O I：
10.1073/pnas.91.24.11621
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Unfixed chicken erythrocyte chromatin fibers in very low salt have been imaged with a scanning force microscope operating in the tapping mode in air at ambient humidity. These images reveal a three dimensional organization of the fibers. The planar ''zig-zag'' conformation is rare, and extended ''beads-on-a-string'' fibers are seen only in chromatin depleted of histones H1 and H5. Glutaraldehyde fixation reveals very similar structures. Fibers fixed in 10 mM salt appear somewhat more compacted, These results, when compared with modeling studies, suggest that chromatin fibers may exist as irregular three-dimensional arrays of nucleosomes even at low ionic strength.
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页码：11621 / 11625
页数：5
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