STRUCTURE OF DROSOPHILA-VIRILIS GLYCEROL-3-PHOSPHATE DEHYDROGENASE GENE AND A COMPARISON WITH THE DROSOPHILA-MELANOGASTER GENE

被引：16

作者：

TOMINAGA, H

SHIBA, T

NARISE, S

机构：

[1] JOSAI UNIV,FAC SCI,BIOL LAB,SAKADO,SAITAMA 35002,JAPAN

[2] KITASATO UNIV,SCH HYG SCI,MOLEC BIOL LAB,SAGAMIHARA,KANAGAWA 228,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1131卷 / 02期

关键词：

GLYCEROL-3-PHOSPHATE DEHYDROGENASE GENE; DNA SEQUENCE; (DROSOPHILA-VIRILIS); (DROSOPHILA-MELANOGASTER);

D O I：

10.1016/0167-4781(92)90086-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete glycerol-3-phosphate dehydrogenase gene of Drosophila virilis isolated by screening with alpha-GPDH(M) cDNA of the adult fly was sequenced. The gene contains eight exons spread over a total of approximate 8 kb DNA. Its exon/intron organization is identical to that of D. melanogaster. A single transcription initiation site was determined by primer extension. The stop codons are located at the 3' end of each of the exons 6 to 8. TATA and CAAT boxes are present upstream of the transcriptional start site. Adult alpha-GPDH protein is encoded by exons 1 to 6 and exon 8. Comparison of the sequence with that of D. melanogaster showed that the homology of the nucleotide sequence of the coding region is 85% and that the homology of the amino acid sequence is 98%. On the contrary, the non-coding region is quite different in length and nucleotide sequence.

引用

页码：233 / 238

页数：6

共 18 条

[1] THE COMPLETE AMINO-ACID-SEQUENCE OF CYTOPLASMIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE FROM DROSOPHILA-VIRILIS [J].