CHROMATOGRAPHIC CHARACTERIZATION OF INTERNAL POLAR LIPIDS FROM WOOL

被引：41

作者：

CODERCH, L ^{[1
]}

DELAMAZA, A ^{[1
]}

SORIANO, C ^{[1
]}

ERRA, P ^{[1
]}

PARRA, JL ^{[1
]}

机构：

[1] CSIC,CID,E-08034 BARCELONA,SPAIN

来源：

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 1995年 / 72卷 / 06期

关键词：

CERAMIDES; GC/MS; POLAR LIPIDS; TLC/FID; WOOL;

D O I：

10.1007/BF02635661

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Wool internal polar lipids were isolated and separated into different fractions based on polarity. Qualitative and quantitative analyses of the different fractions were performed by thin-layer chromatography and thin-layer chromatography coupled to flame-ionization detection, respectively. Cholesterol esters, free fatty acids, sterols, ceramides, glycosylceramides, and cholesterol sulfate were the main components, with ceramides being in the highest proportion. The fatty acid composition of ceramides and glycosylceramides was determined by gas chromatography/mass spectrometry. As for other keratinized tissues, long-chain fatty acids predominated in comparison to either free fatty acids or phospholipid-linked fatty acids; in both cases, stearic and lignoceric acids were the most abundant fatty acids, and a low amount of 18-methyleicosanoic acid was found. This work opens new avenues in the study of lipid rearrangement in more complex and realistic vesicle structures than conventional liposomes.

引用

页码：715 / 720

页数：6

共 38 条

[1]

Ackman R. G., 1990, JPC-J PLANAR CHROMAT, V3, P450

[2] SKIN LIPIDS [J].