DISTRIBUTION OF FREE D-AMINO ACIDS IN THE TISSUES OF CRUSTACEANS

被引：53

作者：

OKUMA, E ^{[1
]}

FUJITA, E ^{[1
]}

AMANO, H ^{[1
]}

NODA, H ^{[1
]}

ABE, H ^{[1
]}

机构：

[1] MIE UNIV,FAC BIORESOURCES,TSU,MIE 514,JAPAN

来源：

FISHERIES SCIENCE | 1995年 / 61卷 / 01期

关键词：

D-ALANINE; D-ASPARTIC ACID; D-AMINO ACID; CRUSTACEAN; (+)-1-(9-FLUORENYL)ETHYL CHLOROFORMATE;

D O I：

10.2331/fishsci.61.157

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Free D-amino acids were determined in the tissues of crustaceans, three macrurans and four brachyurans, using reversed-phase ion-pair HPLC after derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate. Of several D-amino acids detected, D-alanine was the most abundant and widely distributed among every tissue of crustacean species determined. The content of D-alanine in the muscle of these crustaceans ranged from 3.2 to 16.8 mumol/g wet weight. The percentage of D-alanine to total alanine reached 31.7% on average. The second predominant D-amino acid was D-arginine which was less than 2 mumol/g and accounted for less than 10% of the total arginine. Acidic amino acids, D-aspartate and D-glutamate, were present only in the tissues of macrurans and the contents were less than 1 mumol/g. The percentage of D-aspartate to total aspartate, however, varied from 12 to 70%. Several other D-amino acids were also detected in small quantities and low percentages.

引用

页码：157 / 160

页数：4

共 20 条

[1]

ASAKAWA A, 1981, NIPPON SHOKUHIN KOGY, V28, P594

[2] EFFECT OF DIETARY D-AMINO ACIDS ON GROWTH, SURVIVAL, AMMONIA EXCRETION AND SPECIFIC DYNAMIC ACTION IN THE SUPRALITTORAL ISOPOD, LIGIA-PALLASII [J].