HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 2-LTR CIRCLES RESIDE IN A NUCLEOPROTEIN COMPLEX WHICH IS DIFFERENT FROM THE PREINTEGRATION COMPLEX

被引：58

作者：

BUKRINSKY, M ^{[1
]}

SHAROVA, N ^{[1
]}

STEVENSON, M ^{[1
]}

机构：

[1] UNIV NEBRASKA, MED CTR, DEPT PATHOL, OMAHA, NE 68198 USA

来源：

JOURNAL OF VIROLOGY | 1993年 / 67卷 / 11期

关键词：

D O I：

10.1128/JVI.67.11.6863-6865.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The preintegration complex of human immunodeficiency virus type 1 (HIV-1) is a large nucleoprotein complex containing viral nucleic acids in association with products of the viral gag and pol genes. One of these proteins, integrase, is absolutely required for the integration and formation of the provirus. Although HIV-1-specific 2-LTR circles from nuclei of HIV-1-infected cells were found to be associated within a high-molecular-weight nucleoprotein complex, antibodies to HIV-1 integrase failed to precipitate this form of viral DNA. This result indicates that circular forms of HIV-1 DNA are not associated with integrase. These viral DNA forms seem to exist in a context of a nucleoprotein complex that is different from a preintegration complex of HIV-1.

引用

页码：6863 / 6865

页数：3

共 20 条

[1] A NUCLEOPROTEIN COMPLEX MEDIATES THE INTEGRATION OF RETROVIRAL DNA [J].

BOWERMAN, B ;

BROWN, PO ;

BISHOP, JM ;

VARMUS, HE .

GENES & DEVELOPMENT, 1989, 3 (04) :469-478

[2] RETROVIRAL INTEGRATION - STRUCTURE OF THE INITIAL COVALENT PRODUCT AND ITS PRECURSOR, AND A ROLE FOR THE VIRAL IN PROTEIN [J].

BROWN, PO ;

BOWERMAN, B ;

VARMUS, HE ;

BISHOP, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2525-2529

[3]

BROWN PO, 1990, CURR TOP MICROBIOL, V157, P19

[4] ASSOCIATION OF INTEGRASE, MATRIX, AND REVERSE-TRANSCRIPTASE ANTIGENS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 WITH VIRAL NUCLEIC-ACIDS FOLLOWING ACUTE INFECTION [J].

BUKRINSKY, MI ;

SHAROVA, N ;

MCDONALD, TL ;

PUSHKARSKAYA, T ;

TARPLEY, WG ;

STEVENSON, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (13) :6125-6129

[5] ACTIVE NUCLEAR IMPORT OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 PREINTEGRATION COMPLEXES [J].

BUKRINSKY, MI ;

SHAROVA, N ;

DEMPSEY, MP ;

STANWICK, TL ;

BUKRINSKAYA, AG ;

HAGGERTY, S ;

STEVENSON, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (14) :6580-6584

[6] DETERMINATION OF VIRAL-PROTEINS PRESENT IN THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 PREINTEGRATION COMPLEX [J].

FARNET, CM ;

HASELTINE, WA .

JOURNAL OF VIROLOGY, 1991, 65 (04) :1910-1915

[7] CIRCULARIZATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 DNA INVITRO [J].

FARNET, CM ;

HASELTINE, WA .

JOURNAL OF VIROLOGY, 1991, 65 (12) :6942-6952

[8] RETROVIRAL DNA INTEGRATION - STRUCTURE OF AN INTEGRATION INTERMEDIATE [J].

FUJIWARA, T ;

MIZUUCHI, K .

CELL, 1988, 54 (04) :497-504

[9] ANALYSIS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 LTR-LTR JUNCTIONS IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS OF INFECTED INDIVIDUALS [J].

JURRIAANS, S ;

DERONDE, A ;

DEKKER, J ;

GOUDSMIT, J ;

CORNELISSEN, M .

JOURNAL OF GENERAL VIROLOGY, 1992, 73 :1537-1541

[10] THE PALINDROMIC LTR-LTR JUNCTION OF MOLONEY MURINE LEUKEMIA-VIRUS IS NOT AN EFFICIENT SUBSTRATE FOR PROVIRAL INTEGRATION [J].

LOBEL, LI ;

MURPHY, JE ;

GOFF, SP .

JOURNAL OF VIROLOGY, 1989, 63 (06) :2629-2637

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