Confocal/two-photon microscopy in studying colonisation of cancer cells in bone using xenograft mouse models

被引：8

作者：

Allocca, Gloria ^{[1
]}

Kusumbe, Anjali P. ^{[2
]}

Ramasamy, Saravana K. ^{[3
,4
]}

Wang, Ning ^{[1
]}

机构：

[1] Univ Sheffield, Dept Oncol & Metab, Mellanby Ctr Bone Res, Beech Hill Rd, Sheffield S10 2RX, S Yorkshire, England

[2] Univ Oxford, Kennedy Inst Rheumatol, Oxford, England

[3] Imperial Coll London, Inst Clin Sci, Integrat Skeletal Physiol Grp, London, England

[4] MRC, Ctr Clin Sci, Du Cane Rd, London, England

来源：

BONEKEY REPORTS | 2016年 / 5卷

关键词：

D O I：

10.1038/bonekey.2016.84

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Confocal and two-photon microscopy has been widely used in bone research to not only produce high quality, three-dimensional images but also to provide valuable structural and quantitative information. In this article, we describe step-by-step protocols for confocal and two-photon microscopy to investigate earlier cellular events during colonisation of cancer cells in bone using xenograft mouse models. This includes confocal/two-photon microscopy imaging of paraformaldehyde fixed thick bone sections and frozen bone samples.

引用

页数：8

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