A NOVEL PEPTIDE WHICH STIMULATES ADENYLATE-CYCLASE - MOLECULAR-CLONING AND CHARACTERIZATION OF THE OVINE AND HUMAN CDNAS

被引：318

作者：

KIMURA, C

OHKUBO, S

OGI, K

HOSOYA, M

ITOH, Y

ONDA, H

MIYATA, A

JIANG, L

DAHL, RR

STIBBS, HH

ARIMURA, A

FUJINO, M

机构：

[1] TULANE UNIV,HEBERT CTR,US JAPAN BIOMED RES LABS,BELLE CHASSE,LA 70037

[2] TULANE UNIV,SCH MED,DEPT MED,NEW ORLEANS,LA 70112

[3] TULANE UNIV,SCH MED,DEPT PHYSIOL,NEW ORLEANS,LA 70112

[4] TULANE UNIV,SCH MED,DEPT ANAT,NEW ORLEANS,LA 70112

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 166卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91914-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel neuropeptide which remarkably stimulates adenylate cyclase in rat anterior pituitary cell cultures has been recently isolated from ovine hypothalami by A. Arimura and his collaborators (Biochem. Biophys. Res. Commun. 164, 567-574(1989)). This peptide was designated as PACAP3((Pituitary Adenylate Cyclase Activating Polypeptide with 38 residues). In an attempt to investigate physiological implications of PACAP38, we have succeeded in cloning the cDNAs encoding the precursor of PACAP38 from ovine hypothalamus and human testis. An ovine cDNA encodes a protein of 176 amino acids in which PACAP38 is proceeded by a putative signal peptide and a "pro"-region (107 amino acids), and followed by a Gly-Arg-Arg sequence for proteolytic processing and amidation. Deduced amino-acid sequence of human PACAP38 was completely identical to that of the ovine isolated peptide. Cloning of PACAP38 cDNAs confirms the expression of the corresponding mRNAs and the presence of this neuropeptide in ovine hypothalamus and also in human testis. © 1990.

引用

页码：81 / 89

页数：9

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