3D 6DOF Manipulation of Microbead by Laser Tweezers

Laser tweezers are suitable for manipulation of a single microscopic biological object. It can manipulate micro bio-object by noncontact in closed space. Single cell manipulation is important for biological research works, and 3D 6DOF manipulation (Position control and Orientation control) is useful technique in many biological experiments. Here we proposed 3D synchronized laser manipulation system by which we can manipulate multiple micro-objects along each designed trajectory in 3D space. Position and orientation of microbeads can be controlled by the newly developed 3D synchronized laser micromanipulation system. We succeeded in the orientation control of the microbead by using the laser trapped microtools. We demonstrate 3D 6DOF manipulation of the microbead by the experiment.