RAPID AND MICRO HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC DETERMINATION OF CHLORAMPHENICOL IN PLASMA

被引：19

作者：

PENG, GW

GADALLA, MAF

CHIOU, WL

机构：

[1] UNIV ILLINOIS,CLIN PHARMACOKINET LAB,CHICAGO,IL 60612

[2] UNIV ILLINOIS,DEPT PHARM,CHICAGO,IL 60612

来源：

JOURNAL OF PHARMACEUTICAL SCIENCES | 1978年 / 67卷 / 07期

关键词：

Antibacterial–antirickettsials—chloramphenicol; high‐pressure liquid chromatographic analysis in plasma; Chloramphenicol—high‐pressure liquid chormatographic analysis in plasma; High‐pressure liquid chromatography—analysis; chloramphenicol in plasma;

D O I：

10.1002/jps.2600670750

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A high‐pressure liquid chromatographic method was developed for chloramphenicol in plasma. Plasma samples were deproteinized with 2.5 volumes of acetonitrile, and the supernates were chromatographed on a reversed‐phase column, using acidified ethanol–water as the mobile phase and UV spectrophotometry for detection. The sensivity for accurate quantitation of chloramphenicol was about 2.5 μg/ml in plasma, and concentrations as low as 0.5 μg/ml could be detected. Only about 8 min is needed for each sample. This method is specific, rapid, and sufficiently sensitive and may be useful for clinical monitoring. Copyright © 1978 Wiley‐Liss, Inc., A Wiley Company

引用

页码：1036 / 1038

页数：3

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