CELL-SPECIFIC EXPRESSION OF HUMAN BETA-GALACTOSIDE ALPHA-2,6-SIALYTRANSFERASE TRANSCRIPTS DIFFERING IN THE 5' UNTRANSLATED REGION

被引:64
作者
AASHEIM, HC
AASENG, DA
DEGGERDAL, A
BLOMHOFF, HK
FUNDERUD, S
SMELAND, EB
机构
[1] Department of Immunology, Norwegian Radium Hospital, Oslo
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1993年 / 213卷 / 01期
关键词
D O I
10.1111/j.1432-1033.1993.tb17783.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In humans, two cDNAs have been isolated encoding beta-galactoside alpha2,6-sialyltransferase, differing only in part of the 5' untranslated region. Primer extension data show that the two cDNAs are near full-length clones. RNase protection analysis of different cell types showed that the transcript corresponding to the alpha2,6-sialyltransferase cDNA isolated from a B-cell library resided only in mature B cells. In contrast, the transcript corresponding to the alpha2,6-sialyltransferase cDNA isolated from a placenta library was found in all cells tested. Our results also indicate the existence of a third alpha2,6-sialyltransferase transcript in the hepatoma cell line HepG2. Mature B cells were found to express high amounts of alpha2,6-sialyltransferase mRNA, compared to other cell types tested, as shown by Northern blot analysis. Moreover there was an increased expression of beta-galactoside alpha2,6-sialyltransferase mRNA in activated B cells compared to resting B cells. In vitro transcription and translation of the cDNAs resulted in a protein of 45 kDa, but the transcripts were translated with different efficiency, suggesting a role for the 5' untranslated region in regulation of translation. We have also made an alpha2,6-sialyltransferase construct lacking the specific 5' regions of the two cDNAs. A transcript generated from this construct was translated more efficiently in vitro than the two alpha2,6-sialyltransferase cDNAs.
引用
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页码:467 / 475
页数:9
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