SEQUENCE OF A CDNA CLONE ENCODING THE ENZYME MYROSINASE AND EXPRESSION OF MYROSINASE IN DIFFERENT TISSUES OF BRASSICA-NAPUS

被引：46

作者：

FALK, A

XUE, JP

LENMAN, M

RASK, L

机构：

[1] Department of Cell Research, Swedish University of Agricultural Sciences, The Genetic Center, S-750 07 Uppsala

来源：

PLANT SCIENCE | 1992年 / 83卷 / 02期

基金：

瑞典研究理事会;

关键词：

BRASSICA-NAPUS; MYROSINASE; CDNA SEQUENCE; AMINO ACID SEQUENCE; NORTHERN BLOT; RNA SLOTBLOT;

D O I：

10.1016/0168-9452(92)90077-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated and sequenced a cDNA clone for the glucosinolate-degrading enzyme myrosinase from Brassica napus (oilseed rape). Using a probe derived from the cDNA clone we have studied the expression of myrosinase genes in different tissues of B. napus. The myrosinase genes are most highly expressed in the young leaves, the cotyledons and the developing seed. Other parts of the plant only show a relatively low expression of myrosinase. In the developing seed the expression of myrosinase is first detected at 15 days after pollination and at the highest levels around 30 days after pollination. The myrosinase genes are turned on earlier, but also silenced earlier than the genes for the storage protein cruciferin.

引用

页码：181 / 186

页数：6

共 19 条

[1] PURIFICATION, CHARACTERIZATION AND PARTIAL AMINO-ACID SEQUENCING OF BETA-THIOGLUCOSIDASE FROM BRASSICA-NAPUS L [J].