SENSITIVE STEREOSPECIFIC ASSAY OF WARFARIN IN PLASMA - REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION USING DIASTEREOISOMERIC ESTERS OF (-)-(1S,2R,4R)-ENDO-1,4,5,6,7,7-HEXACHLOROBICYCLO[2.2.1]-HEPT-5-ENE-2-CARBOXYLIC ACID

被引：13

作者：

CARTER, SR ^{[1
]}

DUKE, CC ^{[1
]}

CUTLER, DJ ^{[1
]}

HOLDER, GM ^{[1
]}

机构：

[1] UNIV SYDNEY, DEPT PHARM, SYDNEY, NSW 2006, AUSTRALIA

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 574卷 / 01期

关键词：

D O I：

10.1016/0378-4347(92)80100-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A stereospecific reversed-phase high-performance liquid chromatographic (HPLC) assay for warfarin in plasma has been developed. The assay involves a rapid, simple clean-up procedure which separates warfarin from plasma constituents and warfarin metabolites. Warfarin enantiomers were assayed as their (-)-(1S,2R,4R)-endo-1,4,5,6,7,7-hexachlorobicyclo[2.2.1]hept-5-ene-2-carboxylic acid diastereoisomeric esters by reversed-phase HPLC. Excellent resolution of the diastereoisomers was achieved in less than 10 min. Sensitivity of the assay was approximately 5-10 ng/ml for each isomer.

引用

页码：77 / 83

页数：7

共 6 条

[1] ANALYSIS OF WARFARIN ENANTIOMERS - CHROMATOGRAPHIC-SEPARATION OF 6 STEREOISOMERIC ESTERS PREPARED FROM (-)-(1S,2R,4R)-ENDO-1,4,5,6,7,7-HEXACHLOROBICYCLO[2,2,1]HEPT-5-ENE-2-CARBOXYLIC ACID
DUKE, CC
CARTER, SR
HAMBLEY, TW
HOLDER, GM
CUTLER, DJ
CHIRALITY, 1994, 6 (08) : 670 - 680
[2] ENDO-1,4,5,6,7,7-HEXACHLOROBICYCLO[2.2.1]HEPT-5-ENE-2-CARBOXYLIC ACID, A SUPERIOR RESOLVING AGENT FOR THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF ENANTIOMERS OF HYDROXYLATED DERIVATIVES OF 2 AZAAROMATIC HYDROCARBONS
DUKE, CC
HOLDER, GM
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1988, 430 (01): : 53 - 64
[3] APPLICATION OF PUMMERER REACTION TO CHIRAL SULFOXIDE-ESTERS LEADING TO CHIRAL BETA-KETOESTERS - (+)-(1S,2S,4R) AND (-)-(1R,2R,4S) METHYL 3-OXOBICYCLO[2.2.1]HEPT-5-ENE-EXO-2-CARBOXYLATES
GUESSOUS, AR
MAIGNAN, C
BULLETIN DE LA SOCIETE CHIMIQUE DE FRANCE, 1988, (04): : 727 - 730
[4] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF THE PENEM ANTIBIOTIC (5R,6S)-2-AMINOMETHYL-6-[(1R)-HYDROXYETHYL]-2-PENEM-3-CARBOXYLIC ACID IN HUMAN-PLASMA AND URINE
GODBILLON, J
DUVAL, M
GAURON, S
DUBOIS, JP
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1988, 427 (02): : 269 - 276
[5] RAPID, HIGH-SENSITIVITY REVERSED-PHASE LIQUID-CHROMATOGRAPHIC ASSAY FOR 9-CHLORO-2-(2-FURYL)[1,2,4]TRIAZOLO[1,5-C]QUINAZOLIN-5-IMINE AND ITS OXO METABOLITE IN PLASMA USING FLUORESCENCE DETECTION
BRUBAKER, WF
WILLIAMS, ED
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 496 (02): : 377 - 386
[6] DETERMINATION OF (2S, 3S, 5R)-3-METHYL-7-OXO-3-(1H-1,2,3-TRIAZOL-1-YLMETHYL)-4-THIA-1-AZABICYCLO[3.2.0]HEPTANE-2-CARBOXYLIC ACID 4,4-DIOXIDE (YTR-830H) AND PIPERACILLIN IN PHARMACEUTICAL PREPARATIONS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
TSUKAMOTO, T
USHIO, T
JOURNAL OF CHROMATOGRAPHY A, 1994, 678 (01) : 69 - 76

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