SURVEY OF CYTOTOXIN PRODUCTION AMONG ESCHERICHIA-COLI STRAINS CHARACTERIZED AS ENTEROPATHOGENIC (EPEC) BY SEROTYPING AND PRESENCE OF EPEC ADHERENCE FACTOR (EAF) SEQUENCES

被引：23

作者：

GUTH, BEC ^{[1
]}

GIRALDI, R ^{[1
]}

GOMES, TAT ^{[1
]}

MARQUES, LRM ^{[1
]}

机构：

[1] ESCOLA PAULISTA MED,DEPT MICROBIOL IMMUNOL & PARASITOL,BR-04032062 SAO PAULO,BRAZIL

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1994年 / 40卷 / 05期

关键词：

ENTEROPATHOGENIC ESCHERICHIA COLI; EPEC ADHERENCE FACTOR SEQUENCES; SHIGA-LIKE TOXIN; CYTOLETHAL DISTENDING TOXIN;

D O I：

10.1139/m94-056

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A total of 108 Escherichia coli strains characterized as enteropathogenic (EPEC) by serotyping and the presence of EPEC adherence factor (EAF) sequences were examined for cytotoxin production by cell line assays and colony hybridization with Shiga-Like toxin (SLT) probes. Cytolethal distending toxin (CLDT) production was found in three (2.8%) strains belonging to serotype O86:H34, while one O111ab:NM strain hybridized with a SLT-II probe but did not express any cytotoxic activity. All four strains showed localized adherence to HeLa cells and hybridized to an E. coli attaching-effacing gene (eae) probe. The CLDT-producing strains had multiple plasmids and some were present in all strains, including a plasmid of similar to 54 MDa that hybridized with the EAF probe.

引用

页码：341 / 344

页数：4

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