ELECTRON-MICROSCOPIC LOCALIZATION OF RIBOSOMAL DNA IN RAT-LIVER NUCLEOLI BY NONISOTOPIC IN-SITU HYBRIDIZATION

被引：54
作者：
JIMENEZGARCIA, LF
SEGURAVALDEZ, MD
OCHS, RL
ECHEVERRIA, OM
VAZQUEZNIN, GH
BUSCH, H
机构：
[1] SCRIPPS RES INST, WM KECK AUTOIMMUNE DIS CTR, LA JOLLA, CA 92037 USA
[2] BAYLOR COLL MED, DEPT PHARMACOL, HOUSTON, TX 77030 USA
来源：
EXPERIMENTAL CELL RESEARCH | 1993年 / 207卷 / 02期
关键词：
D O I：
10.1006/excr.1993.1186
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
We have used postembedding nonisotopic in situ hybridization, with biotinylated rat ribosomal DNA (rDNA) as a probe and streptavidin coupled to 10-nm colloidal gold particles as the detection system, to localize rDNA sequences in rat liver nucleoli at the electron microscopic level. For comparison purposes, immunoelcctron microscopy was performed for the detection of DNA. Our results indicate that ribosomal DNA sequences are enriched in the dense fibrillar component of the rat liver nucleolus. These data are discussed in relation to the putative site(s) for transcription of ribosomal genes. © 1993 Academic Press, Inc.
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页码：220 / 225
页数：6
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