CATABOLISM OF CYSTEINE, CYSTINE, CYSTEINESULFINATE, AND OTC BY ISOLATED-PERFUSED RAT HINDQUARTER

被引：17

作者：

ENSUNSA, JL ^{[1
]}

HIRSCHBERGER, LL ^{[1
]}

STIPANUK, MH ^{[1
]}

机构：

[1] CORNELL UNIV,DIV NUTR SCI,225 SAVAGE HALL,ITHACA,NY 14853

来源：

AMERICAN JOURNAL OF PHYSIOLOGY | 1993年 / 264卷 / 05期

关键词：

SULFATE; TAURINE; 2-OXOTHIAZOLIDINE-4-CARBOXYLATE;

D O I：

10.1152/ajpendo.1993.264.5.E782

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

The metabolism of cysteine and related compounds was investigated in the isolated perfused hindquarter of the rat. An erythrocyte-based perfusion medium was used; use of a perfluorochemical emulsion, FC-43, resulted in apparent chemical oxidation of cysteine, whereas bovine erythrocytes did not appear to contribute significantly to the metabolism of cysteine. Rat skeletal muscle perfused with L-[S-35]cysteine, L-[S-35]cystine, L-[S-35]cysteinesulfinate, or L-2-oxo-[S-35]thiazolidine-4-carboxylate (OTC) for 2 h produced [S-35]sulfate and [S-35]taurine. In all cases, the partitioning of cysteine or cysteinesulfinate between metabolism to taurine and sulfate was similar, suggesting that cysteine metabolism in hindquarter may occur via formation and catabolism of cysteinesulfinate by either cysteinesulfinate decarboxylase or aspartate (cysteine-sulfinate) aminotransferase. However, the activity of cysteine dioxygenase was extremely low, suggesting that the conversion of cysteine to cysteinesulfinate may have been non-enzymatic.

引用

页码：E782 / E789

页数：8

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