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A SIMPLE HPLC METHOD FOR THE ANALYSIS OF BILE-ACIDS
被引:24
作者:
ZHU, XX
BROWN, GR
机构:
[1] Department of Chemistry, McGill University, Montreal PQ H3A 2K6
基金:
加拿大自然科学与工程研究理事会;
关键词:
BILE ACIDS;
REVERSED-PHASE HPLC;
REFRACTIVE INDEX;
HYDROLYSIS;
D O I:
10.1080/00032719008052545
中图分类号:
O65 [分析化学];
学科分类号:
070302 ;
081704 ;
摘要:
A simple reversed-phase HPLC method has been developed for the analysis of various bile acids, conjugated or unconjugated. A differential refractive index detector and C-18 silica column were used with an aqueous-methanol mobile phase. This method is accurate to 0.5 nmol and provides a simple and convenient quantitative determination of bile acids in aqueous buffer solutions.
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页码:2011 / 2018
页数:8
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