BILE ACIDS;
REVERSED-PHASE HPLC;
REFRACTIVE INDEX;
HYDROLYSIS;
D O I:
10.1080/00032719008052545
中图分类号:
O65 [分析化学];
学科分类号:
070302 ;
081704 ;
摘要:
A simple reversed-phase HPLC method has been developed for the analysis of various bile acids, conjugated or unconjugated. A differential refractive index detector and C-18 silica column were used with an aqueous-methanol mobile phase. This method is accurate to 0.5 nmol and provides a simple and convenient quantitative determination of bile acids in aqueous buffer solutions.