MOLECULAR-INTERACTIONS BETWEEN THE PROTEIN PRODUCTS OF THE NEUROGENIC LOCI NOTCH AND DELTA, 2 EGF-HOMOLOGOUS GENES IN DROSOPHILA

被引：628

作者：

FEHON, RG ^{[1
]}

KOOH, PJ ^{[1
]}

REBAY, I ^{[1
]}

REGAN, CL ^{[1
]}

XU, T ^{[1
]}

MUSKAVITCH, MAT ^{[1
]}

ARTAVANISTSAKONAS, S ^{[1
]}

机构：

[1] INDIANA UNIV,DEPT BIOL,BLOOMINGTON,IN 47405

来源：

CELL | 1990年 / 61卷 / 03期

关键词：

D O I：

10.1016/0092-8674(90)90534-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetic analyses have raised the possibility of interactions between the gene products of the neurogenic loci Notch and Delta, each of which encodes a transmembrane protein with EGF homology. To examine the possibility of intermolecular association between the products of these two genes, we studied the effects of their expression on aggregation in Drosophila S2 cells. We find that Notch-expressing cells form mixed aggregates specifically with cells that express Delta and that this process is calcium dependent. In addition, we show that Notch and Delta can associate within the membrane of a single cell, and further, that they form detergent-soluble intermolecular complexes. Our analyses suggest that Notch and Delta proteins interact at the cell surface via their extracellular domains. © 1990.

引用

页码：523 / 534

页数：12

共 52 条

[1] MOLECULAR-GENETICS OF DELTA, A LOCUS REQUIRED FOR ECTODERMAL DIFFERENTIATION IN DROSOPHILA [J].