BRANCH MIGRATION DURING HOMOLOGOUS RECOMBINATION - ASSEMBLY OF A RUVAB-HOLLIDAY JUNCTION COMPLEX IN-VITRO

被引：67
作者：
HIOM, K
WEST, SC
机构：
来源：
CELL | 1995年 / 80卷 / 05期
关键词：
D O I：
10.1016/0092-8674(95)90357-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The RuvA and RuvB proteins of E. coli promote the branch migration or movement of Holliday junctions during genetic recombination and DNA repair. Using small synthetic Holliday junctions in which the crossover point is confined near one end of the DNA molecule, we show that RuvAB-mediated branch migration occurs with a defined polarity. The assembly of RuvA and RuvB on the Holliday junction has been investigated by sedimentation analysis and by DNase I footprinting. We find that RuvA protein binds and protects all four strands of DNA at the crossover point, whereas RuvB protein binds the DNA asymmetrically. The polarity of branch migration is defined by the asymmetric assembly of the RuvAB branch migration complex relative to the junction and is consistent with a model in which RuvAB drives branch migration by passing the DNA through the hexameric rings of RuvB.
引用
收藏
页码：787 / 793
页数：7
相关论文
共 26 条
[1]   EVIDENCE OF ABORTIVE RECOMBINATION IN RUV MUTANTS OF ESCHERICHIA-COLI-K12 [J].
BENSON, F ;
COLLIER, S ;
LLOYD, RG .
MOLECULAR & GENERAL GENETICS, 1991, 225 (02) :266-272
[2]   NUCLEOTIDE SEQUENCING OF THE RUV REGION OF ESCHERICHIA-COLI K-12 REVEALS A LEXA REGULATED OPERON ENCODING 2 GENES [J].
BENSON, FE ;
ILLING, GT ;
SHARPLES, GJ ;
LLOYD, RG .
NUCLEIC ACIDS RESEARCH, 1988, 16 (04) :1541-1549
[3]   OVERPRODUCTION, PURIFICATION, AND ATPASE ACTIVITY OF THE ESCHERICHIA-COLI RUVB PROTEIN INVOLVED IN DNA-REPAIR [J].
IWASAKI, H ;
SHIBA, T ;
MAKINO, K ;
NAKATA, A ;
SHINAGAWA, H .
JOURNAL OF BACTERIOLOGY, 1989, 171 (10) :5276-5280
[4]   ESCHERICHIA-COLI RUVA-PROTEIN AND RUVB-PROTEIN SPECIFICALLY INTERACT WITH HOLLIDAY JUNCTIONS AND PROMOTE BRANCH MIGRATION [J].
IWASAKI, H ;
TAKAHAGI, M ;
NAKATA, A ;
SHINAGAWA, H .
GENES & DEVELOPMENT, 1992, 6 (11) :2214-2220
[5]   SLIDING CLAMPS OF DNA-POLYMERASES [J].
KURIYAN, J ;
ODONNELL, M .
JOURNAL OF MOLECULAR BIOLOGY, 1993, 234 (04) :915-925
[6]   EFFECT OF RUV MUTATIONS ON RECOMBINATION AND DNA-REPAIR IN ESCHERICHIA-COLI-K12 [J].
LLOYD, RG ;
BENSON, FE ;
SHURVINTON, CE .
MOLECULAR AND GENERAL GENETICS, 1984, 194 (1-2) :303-309
[7]   PROCESSING OF RECOMBINATION INTERMEDIATES BY THE RECG AND RUVAB PROTEINS OF ESCHERICHIA-COLI [J].
LLOYD, RG ;
SHARPLES, GJ .
NUCLEIC ACIDS RESEARCH, 1993, 21 (08) :1719-1725
[8]   ATP-DEPENDENT ASSEMBLY OF DOUBLE HEXAMERS OF SV40 T-ANTIGEN AT THE VIRAL ORIGIN OF DNA-REPLICATION [J].
MASTRANGELO, IA ;
HOUGH, PVC ;
WALL, JS ;
DODSON, M ;
DEAN, FB ;
HURWITZ, J .
NATURE, 1989, 338 (6217) :658-662
[9]   HEXAMERIC RINGS OF ESCHERICHIA-COLI RUVB PROTEIN - COOPERATIVE ASSEMBLY, PROCESSIVITY AND ATPASE ACTIVITY [J].
MITCHELL, AH ;
WEST, SC .
JOURNAL OF MOLECULAR BIOLOGY, 1994, 243 (02) :208-215
[10]  
MULLER B, 1993, J BIOL CHEM, V268, P17185
123