KILLING OF HISTOPLASMA-CAPSULATUM BY GAMMA-INTERFERON-ACTIVATED HUMAN MONOCYTE-DERIVED MACROPHAGES - EVIDENCE FOR A SUPEROXIDE ANION-DEPENDENT MECHANISM

被引：30

作者：

BRUMMER, E ^{[1
]}

KURITA, N ^{[1
]}

YOSHIDA, S ^{[1
]}

NISHIMURA, K ^{[1
]}

MIYAJI, M ^{[1
]}

机构：

[1] CHIBA UNIV,PATHOGEN FUNGI & MICROBIAL TOXICOSES RES CTR,CHIBA,JAPAN

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 1991年 / 35卷 / 01期

关键词：

D O I：

10.1099/00222615-35-1-29

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The interaction of human macrophages with the yeast form of the thermally dimorphic fungal pathogen, Histoplasma capsulatum, was studied. Macrophages derived from monocytes by culture in vitro for 3 days ingested H. capsulatum, but were neither fungicidal or fungistatic. In contrast, when monocytes were exposed to human recombinant gamma-interferon (gamma-IFN) during their differentiation into macrophages, those macrophages were able to reduce the number of ingested or adherent cfu of H. capsulatum by 44-75% in 2 h. Activation of macrophages for fungicidal activity by gamma-IFN was dose dependent and 500-1000 units ml were optimal. Antibody to gamma-IFN abrogated the gamma-IFN activation process. Killing of H. capsulatum by activated macrophages in 2-h assays could be inhibited by superoxide dismutase but not by sodium azide.

引用

页码：29 / 34

页数：6

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