6-AMINOHEXANOIC ACID PLASMINOGEN INTERACTIONS STUDIED BY FLUORESCENCE

被引：9

作者：

VUKPAVLOVIC, S ^{[1
]}

GAFNI, A ^{[1
]}

机构：

[1] WEIZMANN INST SCI, DEPT CHEM PHYS, REHOVOT 76100, ISRAEL

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 91卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91974-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Circular polarization of luminescence spectra of human plasminogen and of its derivatives were measured in solutions of ligand-free proteins and with saturating amounts of 6-aminohexanoic acid. Spectroscopic changes induced by the ligand reveal similar perturbations of the binding sites in all the protein derivatives. It is concluded that the gross conformational change induced by 6-aminohexanoic acid binding to the native plasminogen involves changes of sterical relations of entire protein domains. © 1979.

引用

页码：971 / 975

页数：5

共 14 条

[1] PURIFICATION + PROPERTIES OF HUMAN PLASMINOGEN [J].

ALKJAERSIG, N .

BIOCHEMICAL JOURNAL, 1964, 93 (01) :171-+

[2] MEASUREMENT OF BINDING OF ANTIFIBRINOLYTIC AMINO-ACIDS TO VARIOUS PLASMINOGENS [J].

BROCKWAY, WJ ;

CASTELLINO, FJ .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1972, 151 (01) :194-+

[3] ROTATIONAL DIFFUSION ANALYSIS OF CONFORMATIONAL ALTERATIONS PRODUCED IN PLASMINOGEN BY CERTAIN ANTIFIBRINOLYTIC AMINO-ACIDS [J].

CASTELLINO, FJ ;

BROCKWAY, WJ ;

THOMAS, JK ;

LIAO, HT ;

RAWITCH, AB .

BIOCHEMISTRY, 1973, 12 (15) :2787-2791

[4]

MARKUS G, 1978, J BIOL CHEM, V253, P733

[5]

MARKUS G, 1978, J BIOL CHEM, V253, P727

[6]

OKAMOTO S, 1954, Patent No. 770693

[7] STUDIES ON CONFORMATIONAL-CHANGES OF PLASMINOGEN INDUCED DURING ACTIVATION TO PLASMIN AND BY 6-AMINOHEXANOIC ACID [J].

SJOHOLM, I ;

WIMAN, B ;

WALLEN, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1973, 39 (02) :471-479

[8] CIRCULAR-POLARIZATION OF LUMINESCENCE - BIOCHEMICAL AND BIOPHYSICAL APPLICATIONS [J].

STEINBERG, IZ .

ANNUAL REVIEW OF BIOPHYSICS AND BIOENGINEERING, 1978, 7 :113-137

[9] SENSITIVE INSTRUMENT FOR STUDY OF CIRCULAR POLARIZATION OF LUMINESCENCE [J].

STEINBERG, IZ ;

GAFNI, A .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1972, 43 (03) :409-+

[10]

STEINBERG IZ, 1975, CONCEPTS BIOCH FLUOR, P79

← 1 2 →