ZINC-BINDING BY RETROVIRAL INTEGRASE

被引：21

作者：

MCEUEN, AR

EDWARDS, B

KOEPKE, KA

BALL, AE

JENNINGS, BA

WOLSTENHOLME, AJ

DANSON, MJ

HOUGH, DW

机构：

[1] Biochemistry Dept., University of Bath, Bath, BA2 7AY, Claverton Down

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 189卷 / 02期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0006-291X(92)92275-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Zinc binding by integrase from Moloney murine leukemia virus and a protein A fusion protein containing integrase from human immunodeficiency virus type 1 was demonstrated by a zinc blotting technique using 65ZnCl2. Autoradiography revealed a clear band that was absent gels and in immunoblots. This binding activity was retained in the presence of competing divalent cations and was sensitive to oxidation. This is the first demonstration of zinc binding by intact retroviral integrase. © 1992.

引用

收藏

页码：813 / 818

页数：6

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