CRYSTALLIZATION AND PRELIMINARY ELECTRON-DIFFRACTION STUDY TO 3.7 A OF DNA HELIX-DESTABILIZING PROTEIN-GP32STARI

被引：25
作者：
CHIU, W
HOSODA, J
机构：
[1] Donner Laboratory, University of California, Berkeley
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1978年 / 122卷 / 01期
关键词：
D O I：
10.1016/0022-2836(78)90110-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A two-dimensionally large and thin crystal has been obtained from gp32*I, a proteolytically digested product of a DNA helix-destabilizing protein coded by gene 32 in bacteriophage T4. High-resolution electron diffraction patterns (~3.7Å) are recorded from both unstained and stained protein crystals embedded in glucose. The crystal is of orthorhombic space group with a = 62.9 A ̊ and b = 47.3 A ̊. © 1978.
引用
收藏
页码：103 / 107
页数：5
相关论文
共 13 条
[1]  
ALBERTS B, 1977, NUCLEIC ACID PROTEIN, P31
[2]  
ALBERTS B, 1975, DNA SYNTHESIS ITS RE, V3, P241
[3]   T4 BACTERIOPHAGE GENE-32 - A STRUCTURAL PROTEIN IN REPLICATION AND RECOMBINATION OF DNA [J].
ALBERTS, BM ;
FREY, L .
NATURE, 1970, 227 (5265) :1313-&
[4]   UV SENSITIVITY OF SOME EARLY-FUNCTION TEMPERATURE-SENSITIVE MUTANTS OF PHAGE T4 [J].
BALDY, MW .
VIROLOGY, 1970, 40 (02) :272-+
[5]   STUDIES ON STRUCTURE OF INTRACELLULAR BACTERIOPHAGE-T4 DNA [J].
CURTIS, MJ ;
ALBERTS, B .
JOURNAL OF MOLECULAR BIOLOGY, 1976, 102 (04) :793-816
[6]   PHYSIOLOGICAL STUDIES OF CONDITIONAL LETHAL MUTANTS OF BACTERIOPHAGE T4D [J].
EPSTEIN, RH ;
EDGAR, RS ;
SUSMAN, M ;
STEINBERG, CM ;
BOLLE, A ;
KELLENBERGER, E ;
DENHARDT, GH ;
LIELAUSIS, A ;
BOYDELAT.E ;
CHEVALLEY, R .
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1963, 28 :375-+
[7]  
Glaeser R. M., 1975, PHYSICAL ASPECTS ELE, P205
[8]  
JENSEN DE, 1976, J BIOL CHEM, V251, P7215
[9]   T4 GENE 32 PROTEIN MODEL FOR CONTROL OF ACTIVITY OF REPLICATION FORK [J].
MOISE, H ;
HOSODA, J .
NATURE, 1976, 259 (5543) :455-458
[10]   GENETIC EVIDENCE FOR AN ADDITIONAL FUNCTION OF PHAGE-T4 GENE-32 PROTEIN - INTERACTION WITH LIGASE [J].
MOSIG, G ;
BRESCHKIN, AM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (04) :1226-1230
12