GENOME SIZE AND BASE COMPOSITION IN MEDICAGO-SATIVA AND MEDICAGO-TRUNCATULA SPECIES

被引：124

作者：

BLONDON, F ^{[1
]}

MARIE, D ^{[1
]}

BROWN, S ^{[1
]}

KONDOROSI, A ^{[1
]}

机构：

[1] HUNGARIAN ACAD SCI, BIOL RES CTR, INST GENET, H-61701 SZEGED, HUNGARY

来源：

GENOME | 1994年 / 37卷 / 02期

关键词：

MEDICAGO; GENOME SIZE; BASE COMPOSITION; FLOW CYTOMETRY; SYMBIOSIS;

D O I：

10.1139/g94-037

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The genome size (1C value) and base composition of 14 ecotypes of two species of tetraploid and diploid Medicago have been assessed by flow cytometry. These parameters vary both between and within species. The diploid annual Medicago truncatula Gaertn. had the smallest genome of the group studied (which also covered M. sativa L. subsp. sativa, M. sativa L. subsp. caerulea (Less, ex Ledeb.) Schmalh., M. sativa L. subsp. quasifalcata Sinsk., M. sativa L. subsp. X varia (Martyn) Arcangeli; however, its ecotypes revealed substantial intraspecific variation. The smallest M. truncatula genome observed was ecotype 108-1 with 1C = 0.49 pg and 38.1% GC and the largest was Jemalong with 1C = 0.57 pg and 38.6% GC. The degree of polysomaty in these Medicago was low, although in some tissues the frequency of cells with 4C nuclei reached 50%.

引用

页码：264 / 270

页数：7

共 31 条

[1]

Arumuganathan K., 1991, PLANT MOL BIOL REP, V9, P229, DOI DOI 10.1007/BF02672073

[2]

Barker D. G., 1990, Plant Molecular Biology Reporter, V8, P40, DOI 10.1007/BF02668879

[3] NUCLEAR-DNA AMOUNTS IN ANGIOSPERMS [J].

BENNETT, MD ;

SMITH, JB .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1991, 334 (1271) :309-345

[4] NUCLEAR-DNA AMOUNTS IN ANGIOSPERMS [J].

BENNETT, MD ;

SMITH, JB .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1976, 274 (933) :227-274

[5] NUCLEAR-DNA AMOUNTS IN ANGIOSPERMS [J].

BENNETT, MD ;

SMITH, JB ;

HESLOPHARRISON, JS .

PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1982, 216 (1203) :179-199

[6]

BLONDON F, 1967, CR ACAD SCI D NAT, V264, P596

[7]

BROWN SC, 1993, PORTO WORKSHOP FLOW

[8]

BROWN SC, 1991, LAB GUIDE CELLULAR M, P326

[9]

DENARIE J, 1992, MOL SIGNALS PLANT MI, P295

[10] COMPARISON OF 3 DNA FLUOROCHROMES FOR FLOW CYTOMETRIC ESTIMATION OF NUCLEAR-DNA CONTENT IN PLANTS [J].

DOLEZEL, J ;

SGORBATI, S ;

LUCRETTI, S .

PHYSIOLOGIA PLANTARUM, 1992, 85 (04) :625-631

← 1 2 3 4 →