APPLICATION OF ADSORPTION-KINETICS FOR ESTIMATION OF DISSOCIATION-CONSTANTS

被引：5
作者：
BREIER, A
DURISOVA, V
STANKOVICOVA, T
MISLOVICOVA, D
GEMEINER, P
机构：
[1] SLOVAK ACAD SCI,INST HEART RES,CS-80936 BRATISLAVA,CZECHOSLOVAKIA
[2] SLOVAK ACAD SCI,INST CHEM,CS-80936 BRATISLAVA,CZECHOSLOVAKIA
来源：
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1991年 / 22卷 / 03期
关键词：
DISSOCIATION CONSTANT; ADSORPTION; CALMODULIN; LACTATE DEHYDROGENASE; REACTIVE DYE; NEUROLEPTIC; ANESTHETIC; LOCAL;
D O I：
10.1016/0165-022X(91)90067-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Substances such as drugs, as well as special ligands with expressive biospecific properties, all with different affinities, interact with proteins which can be characterized by dissociation constants. The method for estimation of the dissociation constant on the basis of adsorption kinetics was verified for two typical cases: adsorption of lactate dehydrogenase onto bead cellulose derivatized by reactive dyes C.I.2. or C.I.19, and adsorption of different drugs (neuroleptics and local anesthetics) onto calmodulin immobilized on agarose gel. The real equilibrium values obtained by using the complete time-concentration model of adsorption were fitted according to the respective adsorption isotherms by non-linear regression.
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页码：185 / 193
页数：9
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