INSTRUMENTAL IMPROVEMENTS FOR RAPID, DETAILED KINETIC STUDIES OF OXYGENASE ACTIVITY

被引：24

作者：

COOK, HW ^{[1
]}

FORD, G ^{[1
]}

LANDS, WEM ^{[1
]}

机构：

[1] UNIV MICHIGAN,DEPT BIOL CHEM,ANN ARBOR,MI 48109

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 96卷 / 02期

基金：

英国医学研究理事会; 美国国家科学基金会;

关键词：

D O I：

10.1016/0003-2697(79)90591-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The characteristics of an electronic differentiator that facilitates continuous monitoring of changes in the oxygen concentration measured by a polarographic oxygen electrode are described. In addition, some properties of the electrode membranes that increase the sensitivity of the oxygen-sensing electrode are also described. These modifications greatly improve the accuracy and sensitivity of the biological oxygen monitor for use in kinetic studies of complex enzyme systems such as soybean lipoxygenase or prostaglandin cyclooxygenase. The electronic differentiator also may be used in conjunction with other ratemonitoring instruments such as spectrophotometers and pH meters for accurate quantitation of rapidly changing rates. © 1979.

引用

页码：341 / 351

页数：11

共 16 条

[11] EXTREMELY RAPID BURST OF RESPIRATION UPON AERATION OF MITOCHONDRIA - MEASUREMENT OF OXYGEN-TENSION WITH 10-MSEC TIME RESOLUTION [J].