Two-photon lithography and microscopy of 3D hydrogel scaffolds for neuronal cell growth

被引：81

作者：

Accardo, Angelo ^{[1
]}

Blatche, Marie-Charline ^{[1
]}

Courson, Remi ^{[1
]}

Loubinoux, Isabelle ^{[2
]}

Vieu, Christophe ^{[1
,3
]}

Malaquin, Laurent ^{[1
]}

机构：

[1] Univ Toulouse, CNRS, LAAS, F-31400 Toulouse, France

[2] Univ Toulouse, INSERM, Toulouse NeuroImaging Ctr, ToNIC, F-31024 Ups, France

[3] Univ Toulouse, INSA, F-31400 Toulouse, France

来源：

BIOMEDICAL PHYSICS & ENGINEERING EXPRESS | 2018年 / 4卷 / 02期

基金：

欧盟地平线“2020”;

关键词：

direct laser writing; cell scaffold; 3D fabrication; PEGDA hydrogel; neuro2A cell; multi-photon confocal imaging;

D O I：

10.1088/2057-1976/aaab93

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

3D fabrication techniques are rapidly expanding in the field of scaffold development for cell culture and tissue engineering. Herein we report the realization of free-standing PEGDA hydrogel architectures by using two-photon lithography. The morphological and immunofluorescence characterization of neuro2A cells revealed a tridimensional colonization featuring multiple neuritic extensions per cell as well as the expression of ss-tubulin neuronal marker and actin microfilaments. The results open new perspectives in the continuous quest for structured biomaterials able to provide a favorable environment to cells and at the same time not interfering with imaging protocols necessary for a clear scenario of the cell seeding.

引用

页数：8

共 47 条

[11] Two-photon polymerization of hydrogels - versatile solutions to fabricate well-defined 3D structures
Ciuciu, Adina I.
Cywinski, Piotr J.
[J]. RSC ADVANCES, 2014, 4 (85) : 45504 - 45516
[12] Cvetkovica C, 2014, P NATL ACAD SCI US, V111
[13] Hydrogels for tissue engineering: scaffold design variables and applications
Drury, JL
Mooney, DJ
[J]. BIOMATERIALS, 2003, 24 (24) : 4337 - 4351
[14] Photoinitiator-free 3D scaffolds fabricated by excimer laser photocuring
Farkas, Balazs
Dante, Silvia
Brandi, Fernando
[J]. NANOTECHNOLOGY, 2017, 28 (03)
[15] Designing Biomaterials for 3D Printing
Guvendiren, Murat
Molde, Joseph
Soares, Rosane M. D.
Kohn, Joachim
[J]. ACS BIOMATERIALS SCIENCE & ENGINEERING, 2016, 2 (10): : 1679 - 1693
[16] Electrospun scaffolds for tissue engineering of vascular grafts
Hasan, Anwarul
Memic, Adnan
Annabi, Nasim
Hossain, Monowar
Paul, Arghya
Dokmeci, Mehmet R.
Dehghani, Fariba
Khademhosseini, Ali
[J]. ACTA BIOMATERIALIA, 2014, 10 (01) : 11 - 25
[17] 3D Printed Tissue Models: Present and Future
Jang, Jinah
Yi, Hee-Gyeong
Cho, Dong-Woo
[J]. ACS BIOMATERIALS SCIENCE & ENGINEERING, 2016, 2 (10): : 1722 - 1731
[18] Direct laser writing of synthetic poly(amino acid) hydrogels and poly(ethylene glycol) diacrylates by two-photon polymerization
Kapyla, Elli
Sedlacik, Tomas
Aydogan, Dogu Baran
Viitanen, Jouko
Rypacek, Frantisek
Kellomaki, Minna
[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2014, 43 : 280 - 289
[19] Advances in 3D cell culture technologies enabling tissue-like structures to be created in vitro
Knight, Eleanor
Przyborski, Stefan
[J]. JOURNAL OF ANATOMY, 2015, 227 (06) : 746 - 756
[20] Lemma ED, 2017, IEEE T

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