A SINGLE TREATMENT OF RICE SEEDLINGS WITH 5-AZACYTIDINE INDUCES HERITABLE DWARFISM AND UNDERMETHYLATION OF GENOMIC DNA

被引：125

作者：

SANO, H ^{[1
]}

KAMADA, I ^{[1
]}

YOUSSEFIAN, S ^{[1
]}

KATSUMI, M ^{[1
]}

WABIKO, H ^{[1
]}

机构：

[1] INT CHRISTIAN UNIV,DEPT BIOL,TOKYO 181,JAPAN

来源：

MOLECULAR & GENERAL GENETICS | 1990年 / 220卷 / 03期

关键词：

5-Azacytidine; DNA methylation; Dwarf plants; Oryza sativa;

D O I：

10.1007/BF00391751

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A single exposure of germinated rice seeds (Oryza sativa) to either of the DNA demethylating agents 5-azacytidine (azaC) or 5-azadeoxycytidine (azadC) induced dwarf plants. At maturity, seeds treated with azaC exhibited normal morphological characteristics in comparison with untreated controls except that their height (total stem length) was reduced by about 15%. The M1 progeny, obtained by self-fertilization of an azaC-induced dwarf plant, segregated into dwarf (35%) and apparently tall types (65%). The M2 progenies, obtained by self-fertilization of dwarf M1 plants, were also dwarf, while those from tall M1 plants were only tall. Genomic DNA isolated from mature leaves of azaC-treated seeds showed about a 16% reduction in the 5-methylcytosine (m5C) content in comparison with DNA from untreated samples. A similar reduction in the m5C content was also observed in the M1 and M2 progenies. Thus, both undermethylation and dwarfism induced by azaC treatment were heritable. The results suggest that azaC induced demethylation of genomic DNA, which caused an altered pattern of gene expression and consequently a reduction in plant stem length. © 1990 Springer-Verlag.

引用

页码：441 / 447

页数：7

共 26 条

[1] CHANGES IN T-DNA METHYLATION AND EXPRESSION ARE ASSOCIATED WITH PHENOTYPIC VARIATION AND PLANT-REGENERATION IN A CROWN GALL TUMOR LINE [J].