INTERACTIONS OF BERBERINE WITH POLY(A) AND TRANSFER-RNA

被引：62
|
作者：
NANDI, R [1 ]
DEBNATH, D [1 ]
MAITI, M [1 ]
机构：
[1] INDIAN INST CHEM BIOL,CALCUTTA 700032,W BENGAL,INDIA
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1049卷 / 03期
关键词：
Alkaloid-nucleic acid interaction; Berberine; Poly(A); tRNA;
D O I：
10.1016/0167-4781(90)90107-D
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The interaction of berberine chloride with poly(A) and tRNA has been studied by various spectroscopic techniques. Binding parameters determined from spectrophotometric and spectrofluorimetric measurements by Scatchard analysis indicate a very high effective binding capacity of berberine to poly(A) as compared to DNA or tRNA. The circular dichroism studies show that binding of berberine to poly(A) causes a significant change in the circular dichroic spectrum of poly(A) itself, as manifested by (i) a decrease of both positive and negative bands and (ii) appearance of a conservative type of extrinsic circular dichroic spectrum in the wavelength range of 300-400 nm, while it does not cause any significant alteration to the A form structure of tRNA. It is concluded that berberine interacts stronger with poly (A) than DNA or tRNA. The results are interpreted in terms of its reported biological activities. © 1990.
引用
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页码：339 / 342
页数：4
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