EFFECT OF ASCORBIC-ACID ON RELEASE OF ACETYLCHOLINE FROM SYNAPTIC VESICLES PREPARED FROM DIFFERENT SPECIES OF ANIMALS AND RELEASE OF NORADRENALINE FROM SYNAPTIC VESICLES OF RAT-BRAIN

被引：73

作者：

KUO, CH ^{[1
]}

HATA, F ^{[1
]}

YOSHIDA, H ^{[1
]}

YAMATODANI, A ^{[1
]}

WADA, H ^{[1
]}

机构：

[1] OSAKA UNIV,SCH MED,DEPT PHARMACOL 2,OSAKA 530,JAPAN

来源：

LIFE SCIENCES | 1979年 / 24卷 / 10期

关键词：

D O I：

10.1016/0024-3205(79)90341-2

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Low concentrations of L-ascorbic acid caused release of acetylcholine from isolated synaptic vesicles (rat, guinea-pig and rabbit) in the presence of 2mM ATP, 2 mM MgCl2 and 10-5 M CaCl. The half maximum effect was obtained with about 2 to 2.5 ωM L-ascorbic acid, and the effect was inhibited by addition of 1mM EGTA. The release of noradrenaline from rat synaptic vesicles was also enhanced by L-ascorbic acid, but the concentration for half maximal stimulation was about 20 ωM, indicating that noradrenaline release was less sensitive to L-ascorbic acid than acetylcholine release. The physiological function of L-ascorbic acid in the brain is discussed in relation to release of transmitters. © 1979.

引用

页码：911 / 916

页数：6

共 12 条

[1] Choline esters in tissue extracts. [J].