EFFECT OF ASCORBIC-ACID ON RELEASE OF ACETYLCHOLINE FROM SYNAPTIC VESICLES PREPARED FROM DIFFERENT SPECIES OF ANIMALS AND RELEASE OF NORADRENALINE FROM SYNAPTIC VESICLES OF RAT-BRAIN

被引:73
作者
KUO, CH [1 ]
HATA, F [1 ]
YOSHIDA, H [1 ]
YAMATODANI, A [1 ]
WADA, H [1 ]
机构
[1] OSAKA UNIV,SCH MED,DEPT PHARMACOL 2,OSAKA 530,JAPAN
关键词
D O I
10.1016/0024-3205(79)90341-2
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Low concentrations of L-ascorbic acid caused release of acetylcholine from isolated synaptic vesicles (rat, guinea-pig and rabbit) in the presence of 2mM ATP, 2 mM MgCl2 and 10-5 M CaCl. The half maximum effect was obtained with about 2 to 2.5 ωM L-ascorbic acid, and the effect was inhibited by addition of 1mM EGTA. The release of noradrenaline from rat synaptic vesicles was also enhanced by L-ascorbic acid, but the concentration for half maximal stimulation was about 20 ωM, indicating that noradrenaline release was less sensitive to L-ascorbic acid than acetylcholine release. The physiological function of L-ascorbic acid in the brain is discussed in relation to release of transmitters. © 1979.
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页码:911 / 916
页数:6
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