CLONING AND SEQUENCING OF THE XYNA-GENE ENCODING XYLANASE-A OF ASPERGILLUS-KAWACHII

被引：67
作者：
ITO, K
IKEMASU, T
ISHIKAWA, T
机构：
[1] National Research Institute of Brewing, Tokyo 114, 2-6-30, Takinogawa, Kita-ku
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1992年 / 56卷 / 06期
关键词：
D O I：
10.1271/bbb.56.906
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have cloned the xynA gene coding for xylanase A, a major component of the xylanase family, from Aspergillus kawachii. The cDNA was isolated from an A. kawachii cDNA library by immunoscreening using antibody raised against the purified xylanase A protein. Nucleotide sequence analysis of the cDNA showed a 981-bp open reading frame that encoded a protein of 327 amino acid residues. The signal peptide was composed of 25 amino acid residues and the N-terminus of the mature protein was pyroglutamic acid. The transformed yeast with a cloned cDNA produced xylanase. The genomic DNA was arranged as ten exons and nine introns.
引用
收藏
页码：906 / 912
页数：7
相关论文
共 36 条
  • [1] BIELY P, 1988, METHOD ENZYMOL, V160, P536
  • [2] MICROBIAL XYLANOLYTIC SYSTEMS
    BIELY, P
    [J]. TRENDS IN BIOTECHNOLOGY, 1985, 3 (11) : 286 - 290
  • [3] COMPLETE NUCLEOTIDE-SEQUENCE OF THE XYLANASE GENE FROM THE YEAST CRYPTOCOCCUS-ALBIDUS
    BOUCHER, F
    MOROSOLI, R
    DURAND, S
    [J]. NUCLEIC ACIDS RESEARCH, 1988, 16 (20) : 9874 - 9874
  • [4] THE PRIMARY STRUCTURE OF A 1,4-BETA-GLUCAN CELLOBIOHYDROLASE FROM THE FUNGUS TRICHODERMA-REESEI QM-9414
    FAGERSTAM, LG
    PETTERSSON, LG
    ENGSTROM, JA
    [J]. FEBS LETTERS, 1984, 167 (02) : 309 - 315
  • [5] LAMBDA-REPLACEMENT VECTORS CARRYING POLYLINKER SEQUENCES
    FRISCHAUF, AM
    LEHRACH, H
    POUSTKA, A
    MURRAY, N
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1983, 170 (04) : 827 - 842
  • [6] THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE XYLANASE GENE (XYNA) OF BACILLUS-PUMILUS
    FUKUSAKI, E
    PANBANGRED, W
    SHINMYO, A
    OKADA, H
    [J]. FEBS LETTERS, 1984, 171 (02) : 197 - 201
  • [7] DOMAINS IN MICROBIAL BETA-1,4-GLYCANASES - SEQUENCE CONSERVATION, FUNCTION, AND ENZYME FAMILIES
    GILKES, NR
    HENRISSAT, B
    KILBURN, DG
    MILLER, RC
    WARREN, RAJ
    [J]. MICROBIOLOGICAL REVIEWS, 1991, 55 (02) : 303 - 315
  • [8] NUCLEOTIDE-SEQUENCE AND DELETION ANALYSIS OF THE XYLANASE GENE (XYNZ) OF CLOSTRIDIUM-THERMOCELLUM
    GREPINET, O
    CHEBROU, MC
    BEGUIN, P
    [J]. JOURNAL OF BACTERIOLOGY, 1988, 170 (10) : 4582 - 4588
  • [9] COLONY HYBRIDIZATION - METHOD FOR ISOLATION OF CLONED DNAS THAT CONTAIN A SPECIFIC GENE
    GRUNSTEIN, M
    HOGNESS, DS
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (10) : 3961 - 3965
  • [10] Gurr SJ, 1987, GENE STRUCTURE EUKAR, P93
1234