CLONING AND SEQUENCING OF THE XYNA-GENE ENCODING XYLANASE-A OF ASPERGILLUS-KAWACHII

被引：67
作者：
ITO, K
IKEMASU, T
ISHIKAWA, T
机构：
[1] National Research Institute of Brewing, Tokyo 114, 2-6-30, Takinogawa, Kita-ku
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1992年 / 56卷 / 06期
关键词：
D O I：
10.1271/bbb.56.906
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have cloned the xynA gene coding for xylanase A, a major component of the xylanase family, from Aspergillus kawachii. The cDNA was isolated from an A. kawachii cDNA library by immunoscreening using antibody raised against the purified xylanase A protein. Nucleotide sequence analysis of the cDNA showed a 981-bp open reading frame that encoded a protein of 327 amino acid residues. The signal peptide was composed of 25 amino acid residues and the N-terminus of the mature protein was pyroglutamic acid. The transformed yeast with a cloned cDNA produced xylanase. The genomic DNA was arranged as ten exons and nine introns.
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页码：906 / 912
页数：7
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