DETERMINATION OF ANTISERA TITRES USING SINGLE RADIAL IMMUNODIFFUSION METHOD

An exact determination of the antibody potency of precipitating antisera is possible by the method of single radial immunodiffusion. The titre T is defined as the amount of antigen QAg (in mg or m-mole) which reacts with a known volume of antiserum VAS (in ml) equally distributed in agar gel: T = QAg/VAS [mg/ml or m-mole/ml]. Fundamental quantitative relations between the titre and the parameters easily measurable in the test system as well as a description of the experimental procedure for the determintion of the titres of plasma protein antisera are given. © 1969.