A STUDY OF MUSCLE POLYRIBOSOMES AND COPRECIPITITATION OF POLYRIBOSOMES WITH MYOSIN

被引：83

作者：

HEYWOOD, SM

DOWBEN, RM

RICH, A

机构：

[1] Department of Biology, University of Connecticut, Storrs, Conn

[2] Department of Biology, Massachusetts Institute of Technology, Cambridge

来源：

BIOCHEMISTRY | 1968年 / 7卷 / 09期

关键词：

D O I：

10.1021/bi00849a036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyribosomes from chick skeletal muscle were prepared and found to be very active in protein synthesis. The use of high salt concentrations (0.25 M) in the buffers is necessary because myosin coprecipitates with polysomes at low salt concentrations. The coprecipitation is not limited to a particular size class of polysomes. The addition of myosin to polysomes from tissues other than muscle also results in coprecipitation. This phenomenon can be utilized to prepare purified, enriched polysomes. © 1968, American Chemical Society. All rights reserved.

引用

页码：3289 / &

共 20 条

[1] ULTRASTRUCTURE OF DEVELOPING MUSCLE CELLS IN CHICK EMBRYO [J].