INDUCTION OF CHROMOSOMAL-ABERRATIONS WITH BENZON NUCLEASE IN CHINESE-HAMSTER OVARY (CHO) CELLS

被引：4
|
作者：
JOHANNES, C [1 ]
OBE, G [1 ]
机构：
[1] UNIV ESSEN GESAMTHSCH,DEPT GENET,D-45117 ESSEN,GERMANY
来源：
MUTATION RESEARCH LETTERS | 1994年 / 325卷 / 2-3期
关键词：
BENZON NUCLEASE; CHROMOSOME ABERRATION INDUCTION; CHO CELLS;
D O I：
10.1016/0165-7992(94)90010-8
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Benzon nuclease, an endonuclease originating from Serratia marcescens, was tested for its chromosome breaking activity in Chinese hamster ovary cells. Using a permeabilizing method with hypertonic glycerol, benzon nuclease induced chromosomal aberrations in an S-phase independent manner. The frequencies of polycentric chromosomes were correlated with the dose of the enzyme and the intercellular distribution of aberrations was overdispersed.
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页码：113 / 116
页数：4
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