SYNTHESIS, PURIFICATION AND KINETIC-PROPERTIES OF FLUORESCEIN-LABELED PENICILLINS

被引：58

作者：

LAKAYE, B

DAMBLON, C

JAMIN, M

GALLENI, M

LEPAGE, S

JORIS, B

MARCHANDBRYNAERT, J

FRYDRYCH, C

FRERE, JM

机构：

[1] UNIV LIEGE,INST CHIM,ENZYMOL LAB,B-4000 SART,BELGIUM

[2] UNIV LIEGE,CTR INGN PROT,INST CHIM,B-4000 SART,BELGIUM

[3] UNIV CATHOLIQUE LOUVAIN,CHIM ORGAN SYNTH LAB,B-1348 LOUVAIN,BELGIUM

[4] SMITHKLINE BEECHAM PHARMACEUT,BETCHWORTH,SURREY,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1994年 / 300卷

关键词：

D O I：

10.1042/bj3000141

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis and properties of six fluorescein-labelled penicillins are reported. The two isomers of fluoresceyl-glycyl-6-amino-penicillanic acid are probably the best compounds to use for detection of all the penicillin-binding proteins (PBPs) present in a bacterial membrane preparation. However, the derivatives of ampicillin were much more efficient against Enterobacter aerogenes PBP3. The two isomers obtained when a commercial mixture of the two isomers of carboxyfluorescein was used most often exhibited similar properties, but the Streptomyces R61 extracellular DD-peptidase was only efficiently acylated by the 5'-carboxyfluorescein derivative of glycyl-6-aminopenicillanic acid.

引用

页码：141 / 145

页数：5

共 24 条

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