THE EFFECT OF MEXIDOL ON ATRIAL NATRIURETIC PEPTIDE IN LANGENDORF RAT HEART PREPARATION

Atrial natriuretic peptide (ANP) participating in fluid-and-electrolyte balance maintenance in body plays a certain role in pathogenesis of cardiovascular diseases. The effect of medications on its metabolism is understudied. In cardiology, Mexidol an antihypoxic agent of metabolic type, with a cardioprotective effect - has a widespread application. The effect of Mexidol on ANP was studied for the first time. The aim of the investigation was to study the effect of Mexidol on the intensity of cardiomyocyte ANP accumulation and release in rat isolated perfused heart. Materials and Methods. The experiments were carried out on 15 isolated hearts of male Wistar rats perfused according to Langendorf by Crebs-Henseleit solution with Mexidol administration. The intensity of ANP accumulation and release were assessed by the quantitative analysis of immunolabeled atrial myocyte granules under a transmission electron microscope. Results. Mexidol administered at a dose of 25 mg/kg in a perfusion solution enhances ANP accumulation and release processes in atrial myocytes of a rat Langendorf isolated heart, and results in an additional cardioprotective effect. Slight hypoxia promotes ANP synthesis increase and has no impact on peptide release. Conclusion. The analysis of immunolabeled granules showed a positive effect of Mexidol on ANP synthesis and release in a rat Langendorf isolated heart and proved a cardioprotective action of Mexidol. The study of the effects medications have on ANP will enable to show the possibilities of application of their pharmacological effects.