EVIDENCE THAT FIBRILLAR ANCHORING IS ESSENTIAL FOR AZOSPIRILLUM-BRASILENSE CD ATTACHMENT TO SAND

被引:29
作者
BASHAN, Y
MITIKU, G
WHITMOYER, RE
LEVANONY, H
机构
[1] OHIO STATE UNIV,DEPT AGRON,COLUMBUS,OH 43210
[2] OHIO AGR RES & DEV CTR,ELECTRON MICROSCOPY LAB,WOOSTER,OH 44691
[3] WEIZMANN INST SCI,DEPT PLANT GENET,IL-76100 REHOVOT,ISRAEL
关键词
AZOSPIRILLUM; BACTERIAL ADSORPTION; RHIZOSPHERE BACTERIA; SOIL ADSORPTION; SOIL-BACTERIA INTERACTION;
D O I
10.1007/BF00011014
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Inoculation and incubation of wild type Azospirillum brasilense Cd (agg+) in pure quartz sand resulted in cell attachment to sand particles by a network made up of various sizes and shapes of fibrillar material. Inoculation of sand with an aggregate-deficient mutant of strain Cd (agg-) resulted in no detectable fibrillar formation. Initial attachment ratio between agg+ cells and agg- cells was 4:1. However, similar bacterial populations developed in the sand. Rinsing the sand, colonized by either strains, had a greater effect on agg-; decreasing adsorption from 8.1 to 1.4%. Prolonged rinsing entirely desorbed its cells from the sand. Long bacterial incubation in sand decreased the attachment ratio between agg+ and agg- from 3.4:1 to 2.9:1 and decreased desorption (by rinsing) from 10:1 to 6:1. Agitation increased bacterial population size (from 2 x 10(7) to 4 x 10(8) cfu g-1) and decreased the proportion of attachment of agg+ cells (from 29.2 to 9.8%). A decrease in attachment was being of higher magnitude in the non-aggregating mutant (from 5 to 0% adsorption). Protease treatment of sand colonized with either bacteria decreased attachment of agg+ (from 27.4 to 7.1%) and released proteinaceous compound(s) into the sand only in the agg+ strain. Addition of NaEDTA to sand before inoculation, decreased attachment of agg+ (from 24.2 to 14%) but had no effect on agg-. Addition of low amount of clay (montmorillonite) to sand significantly increased adsorption of agg- to the sand particles (from 8.8 to 98.3%). Survival period of agg+ cells in sand was slightly longer than that of agg- cells. It is proposed that bacterial fibrils are essential for anchoring of A. brasilense to sand.
引用
收藏
页码:73 / 83
页数:11
相关论文
共 20 条
[1]  
ALBRECHT SL, 1983, EXPERIENTIA S, V48, P138
[2]  
BASHAN Y, 1988, J GEN MICROBIOL, V134, P2269
[3]  
BASHAN Y, 1988, J GEN MICROBIOL, V134, P1811
[4]  
BASHAN Y, 1987, J GEN MICROBIOL, V133, P3473
[5]   THE FATE OF FIELD-INOCULATED AZOSPIRILLUM-BRASILENSE CD IN WHEAT RHIZOSPHERE DURING THE GROWING-SEASON [J].
BASHAN, Y ;
LEVANONY, H ;
ZIVVECHT, O .
CANADIAN JOURNAL OF MICROBIOLOGY, 1987, 33 (12) :1074-1079
[7]   AN IMPROVED SELECTION TECHNIQUE AND MEDIUM FOR THE ISOLATION AND ENUMERATION OF AZOSPIRILLUM-BRASILENSE [J].
BASHAN, Y ;
LEVANONY, H .
CANADIAN JOURNAL OF MICROBIOLOGY, 1985, 31 (10) :947-952
[8]   CONTRIBUTION OF AZOSPIRILLUM-BRASILENSE CD TO GROWTH OF TOMATO SEEDLINGS IS NOT THROUGH NITROGEN-FIXATION [J].
BASHAN, Y ;
SINGH, M ;
LEVANONY, H .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1989, 67 (08) :2429-2434
[9]  
BASHAN Y, 1990, IN PRESS CAN J MICRO, V36
[10]  
DANIELS SL, 1980, ADSORPTION MICROORGA, P7