The effect of incubation time on capacitation and acrosome reaction of in vitro ovine spermatozoa

被引：0

作者：

Wattimena, J. ^{[1
]}

机构：

[1] Pertanian Univ, Jurusan Peternakan Fak, Jl Ir M Putuhena, Poka Ambon 97233, Indonesia

来源：

JURNAL ILMU TERNAK DAN VETERINER | 2006年 / 11卷 / 04期

关键词：

Ovine Spermatozoa; Incubation; Capacitation; Acrosome Reaction; In Vitro;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The aim of this research was to study the effect of incubation time on capacitation and acrosome reaction of in vitro ovine spermatozoa. Twelve ejaculates from two Garut sheep were collected using artificial vagina and then evaluated macro and microscopically. After semen washing (centrifugation method), semen was diluted in Brackett and Oliphant (BO) medium and then incubated during 6 hours in CO2 incubator at 38.5 degrees C. Evaluation of capacitation and acrosome reaction was conducted on 0 hour, 0.5 hour, 1 hour, 2 hours, 4 hours and 6 hours incubation time. The result showed that incubation time had significant (P<0.05) effect on no capacitation sperm (pattern F), capacitation (pattern B) and acrosome reaction (pattern AR). Incubation time had significantly (P<0.01) affect the subjective motility, life sperm and membrane integrity.

引用

页码：295 / 301

页数：7

共 50 条

[41] The effect of incubation periods under 95% oxygen on the stimulated acrosome reaction and motility of human spermatozoa [J].

Whittington, K ;

Ford, WCL .

MOLECULAR HUMAN REPRODUCTION, 1998, 4 (11) :1053-1057

[42] Effect of platelet-activating factor on motility, plasmalemma integrity, the process of capacitation and acrosome reaction of fresh and cryopreserved boar spermatozoa [J].

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Tobolski, J. .

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[43] Effect of sperm-agglutinating antibodies on sperm capacitation and acrosome reaction [J].

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INTERNATIONAL JOURNAL OF FERTILITY AND MENOPAUSAL STUDIES, 1996, 41 (06) :528-533

[44] Effect of progesterone on bovine sperm capacitation and acrosome reaction [J].

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[45] Xenoestrogenic compounds promote capacitation and an acrosome reaction in porcine sperm [J].

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[46] The in vitro effect of levonorgestrel on the acrosome reaction of human spermatozoa from fertile men [J].

Bahamondes, L ;

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Munuce, MJ ;

Fazano, F ;

Faúndes, A .

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[47] The in vitro effect of emergency contraception doses of levonorgestrel on the acrosome reaction of human spermatozoa [J].

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de Santis, L ;

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[48] Participation of phosphofructokinase, malate dehydrogenase and isocitrate dehydrogenase in capacitation and acrosome reaction of boar spermatozoa [J].

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Cetica, P. D. .

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[49] Bovine Follicular Fluid Derived Extracellular Vesicles Modulate the Viability, Capacitation and Acrosome Reaction of Bull Spermatozoa [J].

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[50] Signaling pathways in sperm capacitation and acrosome reaction [J].

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