PHOTOCLEAVAGE OF DNA - IRRADIATION OF QUINONE-CONTAINING REAGENTS CONVERTS SUPERCOILED TO LINEAR DNA

被引：38
作者：
KOCH, T
ROPP, JD
SLIGAR, SG
SCHUSTER, GB
机构：
[1] UNIV ILLINOIS,DEPT CHEM,URBANA,IL 61801
[2] UNIV ILLINOIS,DEPT BIOCHEM,ROGER ADAMS LAB,URBANA,IL 61801
来源：
PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1993年 / 58卷 / 04期
关键词：
D O I：
10.1111/j.1751-1097.1993.tb04931.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Irradiation (350 nm) of air-saturated solutions of reagents containing an anthraquinone group linked to quaternary alkyl ammonium groups converts supercoiled DNA to circular and to linear DNA. Generation of linear DNA does not occur by accumulation of numerous single-strand cuts but by coincident-site double-strand cleavage of DNA. Irradiation forms the triplet state of the anthraquinone, which reacts either by hydrogen atom abstraction from a sugar of DNA or by electron transfer from a base of the DNA. Subsequent reactions result in chain scission. The quinone is apparently reformed after this sequence and reirradiation leads to double-strand cleavage.
引用
收藏
页码：554 / 558
页数：5
相关论文
共 46 条
[1]   DESIGN OF A SEQUENCE-SPECIFIC DNA-CLEAVING MOLECULE WHICH CONJUGATES A COPPER-CHELATING PEPTIDE, A NETROPSIN RESIDUE, AND AN ACRIDINE CHROMOPHORE [J].
BAILLY, C ;
SUN, JS ;
COLSON, P ;
HOUSSIER, C ;
HELENE, C ;
WARING, MJ ;
HENICHART, JP .
BIOCONJUGATE CHEMISTRY, 1992, 3 (02) :100-103
[2]   EFFECTS OF IONIZING-RADIATION ON DEOXYRIBONUCLEIC-ACID AND RELATED SYSTEMS .1. THE ROLE OF OXYGEN [J].
BOON, PJ ;
CULLIS, PM ;
SYMONS, MCR ;
WREN, BW .
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, 1984, (08) :1393-1399
[3]  
BRAUER WR, 1978, ANN REV BIOCH BIOENG, V7, P287
[4]   9-(4-NITROBENZAMIDOPOLYMETHYLENE)AMINOACRIDINES AND THEIR PHOTOCHEMICAL CLEAVAGE OF DNA [J].
BUCHARDT, O ;
EGHOLM, M ;
KARUP, G ;
NIELSEN, PE .
JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1987, (22) :1696-1697
[5]  
CADET J, 1983, ISRAEL J CHEM, V23, P420
[6]  
CHOUDHURI J, 1986, J INDIAN CHEM SOC, V63, P60
[7]   RADIOLYSIS OF POLY(U) IN OXYGENATED SOLUTION [J].
DEEBLE, DJ ;
VONSONNTAG, C .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1986, 49 (06) :927-936
[8]   SITE-SPECIFIC ATOM TRANSFER FROM DNA TO A BOUND LIGAND DEFINES THE GEOMETRY OF A DNA CALICHEAMICIN GAMMA-1I COMPLEX [J].
DEVOSS, JJ ;
TOWNSEND, CA ;
DING, WD ;
MORTON, GO ;
ELLESTAD, GA ;
ZEIN, N ;
TABOR, AB ;
SCHREIBER, SL .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (26) :9669-9670
[9]   PHOTO-SENSITIZATION OF PYRIMIDINES BY 2-METHYLNAPHTHOQUINONE IN WATER - A LASER FLASH-PHOTOLYSIS STUDY [J].
FISHER, GJ ;
LAND, EJ .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1983, 37 (01) :27-32
[10]   DISSOCIATION KINETICS OF DNA-ANTHRACYCLINE AND DNA-ANTHRAQUINONE COMPLEXES DETERMINED BY STOPPED-FLOW SPECTROPHOTOMETRY [J].
GANDECHA, BM ;
BROWN, JR ;
CRAMPTON, MR .
BIOCHEMICAL PHARMACOLOGY, 1985, 34 (06) :733-736
12345