IDENTIFICATION OF A 1,25-DIHYDROXYVITAMIN-D3-RESPONSE ELEMENT IN THE 5'-FLANKING REGION OF THE RAT CALBINDIN D-9K GENE

被引：173
作者：
DARWISH, HM
DELUCA, HF
机构：
[1] Department of Biochemistry, University of Wisconsin-Madison, 420 Henry Mall, Madison
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 02期
关键词：
HORMONE-RESPONSIVE ELEMENTS; CALCIUM-BINDING PROTEIN GENE;
D O I：
10.1073/pnas.89.2.603
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The rat calbindin D-9k gene is transcriptionally regulated by 1,25-dihydroxyvitamin D3 in the intestine. We have examined the 5'-flanking region of this gene and identified a 1,25-dihydroxyvitamin D3-responsive element (DRE) between nucleotides -489 and -445. This element confers 1,25-dihydroxyvitamin D3 responsiveness through its native promoter and the heterologous thymidine kinase promoter, and it contains the sequence GGGTGTCGGAAGCCC, which is homologous to the other previously identified DREs. Incubation of this element with the 1,25-dihydroxyvitamin D3 receptor produced a specific protein-DNA complex, which shifted to a higher molecular weight form upon the addition of a monoclonal antibody specific to the 1,25-dihydroxyvitamin D3 receptor. Therefore, the 5'-flanking region of the rat calbindin D-9k gene contains a DRE that mediates the enhanced expression of this gene by 1,25-dihydroxyvitamin D3 in the intestine.
引用
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页码：603 / 607
页数：5
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