RANDOM AND SITE-DIRECTED MUTAGENESIS OF BACTERIAL LUCIFERASE - INVESTIGATION OF THE ALDEHYDE BINDING-SITE

被引：24
作者：
CHEN, LH
BALDWIN, TO
机构：
[1] TEXAS A&M UNIV,DEPT BIOCHEM & BIOPHYS,COLLEGE STN,TX 77843
[2] TEXAS AGR EXPT STN,COLLEGE STN,TX 77843
来源：
BIOCHEMISTRY | 1989年 / 28卷 / 06期
关键词：
D O I：
10.1021/bi00432a048
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
引用
收藏
页码：2684 / 2689
页数：6
相关论文
共 25 条
[11]   ISOLATION OF BACTERIAL LUCIFERASES BY AFFINITY-CHROMATOGRAPHY ON 2,2-DIPHENYLPROPYLAMINE-SEPHAROSE - PHOSPHATE-MEDIATED BINDING TO AN IMMOBILIZED SUBSTRATE-ANALOG [J].
HOLZMAN, TF ;
BALDWIN, TO .
BIOCHEMISTRY, 1982, 21 (24) :6194-6201
[12]  
KUNKEL TA, 1987, METHOD ENZYMOL, V154, P367
[13]   RAPID AND EFFICIENT SITE-SPECIFIC MUTAGENESIS WITHOUT PHENOTYPIC SELECTION [J].
KUNKEL, TA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (02) :488-492
[14]  
Maniatis T., 1982, MOL CLONING
[15]  
MEIGHEN EA, 1971, J BIOL CHEM, V246, P7666
[16]   FLAVINE SPECIFICITY OF ENZYME-SUBSTRATE INTERMEDIATES IN BACTERIAL BIOLUMINESCENT REACTION - STRUCTURAL REQUIREMENTS OF FLAVINE SIDE-CHAIN [J].
MEIGHEN, EA ;
MACKENZI.RE .
BIOCHEMISTRY, 1973, 12 (08) :1482-1491
[17]   STABLE, INEXPENSIVE, SOLID-STATE PHOTOMULTIPLIER PHOTOMETER [J].
MITCHELL, GW ;
HASTINGS, JW .
ANALYTICAL BIOCHEMISTRY, 1971, 39 (01) :243-&
[18]  
Nealson K. H., 1978, METHOD ENZYMOL, V57C, P153
[19]  
RAUSCH SK, 1983, THESIS U ILINOIS URB
[20]   DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].
SANGER, F ;
NICKLEN, S ;
COULSON, AR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467
123