RAPID DETECTION OF 1B, 1BL/1RS HETEROZYGOTES IN THE DEVELOPMENT OF HOMOZYGOUS 1BL/1RS TRANSLOCATION STOCKS OF TRITICUM-TURGIDUM (2N=4X=28)

被引：14
作者：
WILLIAM, MDHM
MUJEEBKAZI, A
机构：
来源：
GENOME | 1993年 / 36卷 / 06期
关键词：
TRITICUM TURGIDUM; GLUCOSE PHOSPHATE ISOMERASE; 1BL/1RS TRANSLOCATION; ISOELECTRIC FOCUSING;
D O I：
10.1139/g93-144
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A biochemical marker was utilized to facilitate detection of chromosome 1B, 1BL/1RS translocation heterozygote plants in segregating backcross progenies during the development of 1BL/1RS homozygous lines in several Triticum turgidum L. cultivars (2n = 4x = 28; AABB). Isoelectric focussing of glucose phosphate isomerase (GPI) on either pH 3.5-9.5 or 5.5-8.5 polyacrylamide gels facilitated the detection of 1B, 1BL/1RS translocation heterozygotes from the homozygous 1B or 1BL/1RS derivatives during each backcross of the heterozygote to the respective recurrent parent. The biochemical diagnostic procedure complements the more time consuming and cumbersome chromosome banding technique. This GPI diagnostic in durum 1BL/1RS development is also swifter than a similar stocks development in T. aestivum where both GPI and acid PAGE are essential.
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页码：1088 / 1091
页数：4
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