THE HIGH-SPIN LOW-SPIN EQUILIBRIUM IN CYTOCHROME-P-450 - A NEW METHOD FOR DETERMINATION OF THE HIGH-SPIN CONTENT

被引：45
作者：
JUNG, C
RISTAU, O
REIN, H
机构：
[1] Central Institute of Molecular Biology, the Academy of Sciences, the G.D.R., Berlin
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1076卷 / 01期
关键词：
CYTOCHROME-P-450; ELECTRONIC ABSORPTION SPECTRA; HIGH-SPIN LOW-SPIN EQUILIBRIUM; SPIN STATE;
D O I：
10.1016/0167-4838(91)90229-S
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new method for determination of the population of the high-spin state (high-spin content) in ferric cytochrome P-450 is presented. Based on curve fitting the electronic absorption spectra with a linear combination of gaussian bands analytical functions for the pure high-spin and pure low-spin states were constructed. These functions were used to fit the high-spin/low-spin mixed spectra. A good fit of the absorption spectra of six different cytochrome P-450 proteins in the presence and absence of substrates was found, indicating a similar pi-electron structure of the porphyrin and a similar chemical nature of the nearest coordination sphere of the iron in all cytochrome P-450 proteins.
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页码：130 / 136
页数：7
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