MESSENGER-RNA DEGRADATION IN ESCHERICHIA-COLI - A NOVEL FACTOR WHICH IMPEDES THE EXORIBONUCLEOLYTIC ACTIVITY OF PNPASE AT STEM-LOOP STRUCTURES

被引：52

作者：

CAUSTON, H

PY, B

MCLAREN, RS

HIGGINS, CF

机构：

[1] UNIV OXFORD, JOHN RADCLIFFE HOSP, INST MOLEC MED, IMPERIAL CANC RES FUND LABS, OXFORD OX3 9DU, ENGLAND

[2] UNIV OXFORD, JOHN RADCLIFFE HOSP, NUFFIELD DEPT CLIN BIOCHEM, OXFORD OX3 9DU, ENGLAND

来源：

MOLECULAR MICROBIOLOGY | 1994年 / 14卷 / 04期

关键词：

D O I：

10.1111/j.1365-2958.1994.tb01310.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Stem-loop structures can protect upstream mRNA from degradation by impeding the processive activities of 3'-5' exoribonucleases. The ability of such structures to impede exonuclease activity in vitro is insufficient to account for the stability they can confer on mRNA in vivo. In this study we identify a factor from Escherichia coli which specifically impedes the processive activity of the 3'-5' exonuclease PNPase at stem-loop structures in vitro. This factor can, potentially, reconcile the apparent. discrepancy between the ability of 3' stem-loop structures to stabilize upstream mRNA in vitro and in vivo. Its mechanism of action, and possible role in regulating mRNA degradation, is discussed.

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页码：731 / 741

页数：11

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