Comparison of four DNA extraction methods for forensic application

被引：9

作者：

Bogas, V. ^{[1
]}

Balsa, F. ^{[1
,2
]}

Carvalho, M. ^{[1
,2
]}

Anjos, M. J. ^{[1
,2
]}

Pinheiro, M. F. ^{[2
,3
,4
,5
]}

Corte-Real, F. ^{[2
,6
,7
]}

机构：

[1] Natl Inst Legal Med, Forens Genet Serv, Ctr Branch, Coimbra, Portugal

[2] CENCIFOR, Forens Sci, Coimbra, Portugal

[3] Natl Inst Legal Med, Forens Genet Serv, North Branch, Oporto, Portugal

[4] Univ Fernando Pessoa, Fac Hlth Sci, Oporto, Portugal

[5] Fac Biomed Sci Abel Salazar, Oporto, Portugal

[6] Natl Inst Legal Med, Ctr Branch, Largo Se Nova, P-3000213 Coimbra, Portugal

[7] Univ Coimbra, Med Fac, Coimbra, Portugal

来源：

FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES | 2011年 / 3卷 / 01期

关键词：

DNA extraction; Degraded DNA;

D O I：

10.1016/fsigss.2011.08.097

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Challenging biological samples found in crime scenes are often brought to our lab. Several factors, such as degradation and the presence of inhibitors, can difficult the analysis of these samples. Chelating resin, silica membranes, silica-coated magnetic beads and paramagnetic resin were DNA extraction techniques used in this study. Our aim was to find out the DNA extraction method more suitable to overcome problems raised by these samples and to enable the identification of its genetic profile. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

引用

页码：E194 / E195

页数：2