SEPARATION OF TRANSFER RIBONUCLEIC-ACIDS ON POLYSTYRENE ANION-EXCHANGERS

被引：15
作者：
SINGHAL, RP
GRIFFIN, GD
NOVELLI, GD
机构：
[1] WICHITA STATE UNIV, DEPT CHEM, WICHITA, KS 67208 USA
[2] OAK RIDGE NATL LAB, DIV BIOL, OAK RIDGE, TN 37830 USA
来源：
BIOCHEMISTRY | 1976年 / 15卷 / 23期
关键词：
D O I：
10.1021/bi00668a021
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The tRNA separation by chromatography on strong-base-polystyrene exchange materials is examined and compared with the widely used reversed-phase chromatography. Results indicate important differences in some tRNA elution patterns by the anion-exchange chromatography, as compared with the reversed-phase chromatography. tRNA containing hydrophobic groups are adsorbed more strongly. The anion exchanger has twice the number of theoretical plates. Single peaks of tRNA2Glu and tRNA1Phe obtained from the reversed-phase column give multiple peaks on polystyrene anion-exchange chromatography. All 6 leucine tRNA (Escherichia coli) and differences in tRNA populations synthesized during early and late stages of the dividing lymphocytes from normal human blood can be characterized by the anion-exchange chromatography. Different separation profiles are obtained by 2 separation systems for tyrosine tRNA from mouse liver and mouse-plasma-cell tumor [MOPC-31c]. In contrast to the reversed-phase chromatography, strong-base-polystyrene anion-exchange chromatography is capable of separating tRNA with minor structural differences.
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页码：5083 / 5087
页数：5
相关论文
共 28 条
[11]  
KELMERS AD, 1965, J BIOL CHEM, V240, P3979
[12]  
KELMERS AD, 1971, METHODS ENZYMOLOGY C, V20, P9
[13]   SEPARATION OF LYMPHOCYTES POLYMORPHONUCLEAR LEUKOCYTES + MONOCYTES ON GLASS COLUMNS INCLUDING TISSUE CULTURE OBSERVATIONS [J].
RABINOWITZ, Y .
BLOOD, 1964, 23 (06) :811-&
[14]   ISOLATION AND SEQUENCE ANALYSIS OF TRANSFER-RNA - USE OF PLASKON CHROMATOGRAPHY (RPC-5) [J].
ROE, B ;
MARCU, K ;
DUDOCK, B .
BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 319 (01) :25-36
[15]   STUDIES ON HUMAN TRANSFER-RNA .1. RAPID, LARGE-SCALE ISOLATION AND PARTIAL FRACTIONATION OF PLACENTA AND LIVER TRANSFER-RNA [J].
ROE, BA .
NUCLEIC ACIDS RESEARCH, 1975, 2 (01) :21-42
[16]   DETERMINATION OF TRANSFER RIBONUCLEIC ACID BY AMINOACYLATION .I. LEUCINE AND PHENYLALANINE TRANSFER RIBONUCLEIC ACID FROM E COLI B [J].
RUBIN, IB ;
KELMERS, AD ;
GOLDSTEIN, G .
ANALYTICAL BIOCHEMISTRY, 1967, 20 (03) :533-+
[17]   ANION-EXCHANGE CHROMATOGRAPHY ON REVERSED-PHASE COLUMNS - ISOLATION AND ASSAY OF NUCLEOSIDES, NUCLEOTIDES, AND OLIGONUCLEOTIDES FROM NUCLEIC-ACIDS AND FROM CYTOPLASM [J].
SINGHAL, RP .
BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 319 (01) :11-24
[18]  
SINGHAL RP, 1975, FED PROC, V34, P612
[19]   EXAMINATION OF HIGHLY PURIFIED TRANSFER-RNAS FROM ESCHERICHIA-COLI - DIFFERENCES IN AMOUNT OF MINOR COMPONENTS AND PRESENCE OF A CYTIDINE-THIOURIDINE PHOTOPRODUCT IN NORMAL TRANSFER-RNAS - COMPARISON OF 2 ANALYTICAL METHODS [J].
SINGHAL, RP ;
BEST, AN .
BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 331 (03) :357-368
[20]   CATION-EXCLUSION CHROMATOGRAPHY ON ANION-EXCHANGERS - APPLICATION TO NUCLEIC-ACID COMPONENTS AND COMPARISON WITH ANION-EXCHANGE CHROMATOGRAPHY [J].
SINGHAL, RP ;
COHN, WE .
BIOCHEMISTRY, 1973, 12 (08) :1532-1537
123