EMBRYOGENIC PROTOPLAST CULTURES OF ORANGE JESSAMINE (MURRAYA-PANICULATA) AND THEIR REGENERATION INTO PLANTS FLOWERING IN-VITRO

被引：15

作者：

JUMIN, HB ^{[1
]}

NITO, N ^{[1
]}

机构：

[1] SAGA UNIV,FAC AGR,SAGA 840,JAPAN

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1995年 / 41卷 / 03期

关键词：

FLORAL BUD FORMATION; ORANGE JESSAMINE; PLATING EFFICIENCY; PRECOCIOUS FLOWERING;

D O I：

10.1007/BF00045092

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Embryogenic callus was induced from the hypocotyl region of seedlings germinated from immature embryos of orange jessamine (Murraya paniculata (L.) Jack) on Murashige & Tucker (1969) medium containing 50 g l(-1) sucrose, 5.0 mg l(-1) benzyladenine, 2.5 mg l(-1) 2,4-dichlorophenoxyacetic acid and 600 mg l(-1) malt extract. Isolated protoplasts divided to produce callus on Murashige and Tucker (1969) medium containing 50 g l(-1) sucrose, 0.01 mg l(-1) gibberellin A(4+7) and 600 mg l(-1) malt extract. Callus developed to plantlets via somatic embryogenesis on Murashige and Tucker (1969) medium with 50 g l(-1) lactose but no plant growth regulators. These plantlets flowered in vitro on half strength Murashige and Tucker (1969) medium containing 50 g l(-1) sucrose after 2 months culture.

引用

页码：277 / 279

页数：3

共 9 条

[1]

Bitters W.P., Brusca J.A., Cole D.A., The search for new citrus rootstocks, Calif. Citrograph, 49, pp. 443-448, (1964)

[2]

Grosser J.W., Gmitter F.G., Chandler J.L., Intergeneric somatic hybrid plants from sexually incompatible woody species: Citrus sinensis and Severinia disticha, Theor. Appl. Genet., 75, pp. 397-401, (1988)

[3]

Iwamasa M., Nito N., Ling J.T., Intra- and intergeneric hybridization in the orange subfamily, Aurantioideae, Proc. Int. Soc. Citriculture, 6, pp. 123-130, (1988)

[4]

Murashige T., Tucker D.P.H., Growth factor requirements of citrus tissue culture, Proc. 1st. Citrus Symp., 3, pp. 1155-1161, (1969)

[5]

Nagata T., Takebe I., Cell wall regeneration and cell division in isolated tobacco mesophyl protoplasts, Planta, 92, pp. 301-308, (1970)

[6]

Shinozaki S., Fujita S., Hidaka T., Omura M., Plantlet formation of somatic hybrids of sweet orange (Citrus sinensis) and its wild relative, orange jessamine (Murraya paniculata), by electrically-induced protoplast fusion, Ikushugaku zasshi, 42, pp. 287-295, (1992)

[7]

Swingle W., Reece P.C., The botany of Citrus and its wild relatives, The citrus industry, Vol 1, pp. 190-430, (1967)

[8]

Sykes S.R., An overview of the family Rutaceae, Citrus Breeding Workshop, pp. 93-100, (1988)

[9]

Takayanagi R., Hidaka T., Omura M., Regeneration of intergeneric somatic hybrids by electrical fusion between Citrus and wild relatives: Mexican lime (Citrus aurantifolia) and Java feroniella (Feroniella lucida) or tabog (Swinglea glutinosa), Engei Gakkai zasshi, 60, pp. 799-804, (1992)

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