DETECTION OF A PROTEIN AND DIFFERENCES IN THE POPULATIONS OF TRANSLATABLE MESSENGER-RNA SPECIFIC TO DWARF RICE (ORYZA-SATIVA L)

To isolate genes related to dwarfism in rice, we analysed the differences in the expressions of cellular proteins and translation products of mRNA between normal and dwarf rice of isogenic lines. By analysis of soluble proteins, one protein of 93 kD was detected exclusively in dwarf rices (Akibare-waisei and Hosetsu-waisei). This protein was detected up 2 weeks after imbibition, however it disappeared after 4 weeks. From analysis of in vitro translation products of mRNA by two-dimensional gel electrophoresis, thirty one differences of spots expressed by translatable mRNA were observed among normal rice, Akibare and extreme dwarf rices, Akibare-waisei and Hosetsu-waisei at 2 and 4 weeks after imbibition. In these differences, twenty spots were detected in normal, Akibare and five spots were detected in extreme dwarf rices both Akibare-waisei and Hosetsu-waisei at 2 weeks. Most of the spots specific to normal and dwarf rices almost disappeared at 4 weeks, but two new spots appeared in both normal and dwarf rices.